Sequential entry of components of gene expression machinery into daughter nuclei
Sequential entry of components of gene expression machinery into daughter nuclei
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DOI：
10.1091/mbc.e02-10-0669
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发表时间：
2003-03-01
影响因子：
3.3
通讯作者：
Spector, DL
中科院分区：
文献类型：
--
作者：
Prasanth, KV;Sacco-Bubulya, PA;Spector, DL
在真核细胞中，RNA聚合酶11（RNA poll 11）转录和前mRNA加工是协调事件。我们已经解决了如何在有丝分裂过程中组织转录和前mRNA加工机制的各个组件，并随后招募到新形成的子细胞核。有趣的是，许多RNA聚合酶11转录和前mRNA加工因子的定位研究揭示了这些因子在核被膜/核纤层形成后进入子核的非随机和顺序进入。起始主管形式的RNA聚合酶11和一般的转录因子同时出现在子细胞核中，但在前mRNA加工因子，而延伸主管形式的RNA聚合酶11被检测到，甚至更晚。这些要素的差异进入排除了它们作为单一复合体被运输的可能性。末期细胞核有能力转录和前mRNA剪接伴随着各自的因素的初始进入。此外，我们的研究结果揭示了低周转率的转录和前mRNA剪接因子在有丝分裂。我们提供的证据支持一个模型，其中RNA聚合酶11基因表达机制进入新形成的子细胞核是一个分阶段和有序的过程。
In eukaryotic cells, RNA polymerase 11 (RNA pol 11) transcription and pre-mRNA processing are coordinated events. We have addressed how individual components of the transcription and pre-mRNA processing machinery are organized during mitosis and subsequently recruited into the newly formed daughter nuclei. Interestingly, localization studies of numerous RNA pol 11 transcription and pre-mRNA processing factors revealed a nonrandom and sequential entry of these factors into daughter nuclei after nuclear envelope/lamina formation. The initiation competent form of RNA pol 11 and general transcription factors appeared in the daughter nuclei simultaneously, but prior to pre-mRNA processing factors, whereas the elongation competent form of RNA pol 11 was detected even later. The differential entry of these factors rules out the possibility that they are transported as a unitary complex. Telophase nuclei were competent for transcription and pre-mRNA splicing concomitant with the initial entry of the respective factors. In addition, our results revealed a low turnover rate of transcription and pre-mRNA splicing factors during mitosis. We provide evidence to support a model in which the entry of the RNA pol 11 gene expression machinery into newly forming daughter nuclei is a staged and ordered process.