THE DROSOPHILA PEANUT GENE IS REQUIRED FOR CYTOKINESIS AND ENCODES A PROTEIN SIMILAR TO YEAST PUTATIVE BUD NECK FILAMENT PROTEINS
THE DROSOPHILA PEANUT GENE IS REQUIRED FOR CYTOKINESIS AND ENCODES A PROTEIN SIMILAR TO YEAST PUTATIVE BUD NECK FILAMENT PROTEINS
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DOI：
10.1016/0092-8674(94)90152-x
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发表时间：
1994-05-06
期刊：
影响因子：
64.5
通讯作者：
RUBIN, GM
中科院分区：
文献类型：
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作者：
NEUFELD, TP;RUBIN, GM
我们已经鉴定出一个果蝇基因，花生（pnut），它与酿酒葡萄球菌的CDC3、CDC10、CDC11和CDC12基因序列相关。这些基因是细胞质分裂所必需的，它们的产物在细胞分裂时出现在芽颈上。我们发现pnut也是细胞分裂所必需的：在pnut突变体中，想象组织不能增殖，而是发育成大的多核细胞簇。Pnut蛋白定位于细胞分裂过程中分裂细胞的分裂沟和连接有丝分裂后子细胞的细胞间桥梁。除了在细胞分裂中发挥作用外，pnut还与复眼中决定神经元命运所需的基因7缺失相互作用，这表明pnut可能具有多效性功能。我们的结果表明，这类蛋白质参与了果蝇和酵母之间保守的细胞质分裂的各个方面。
We have identified a Drosophila gene, peanut (pnut), that is related in sequence to the CDC3, CDC10, CDC11, and CDC12 genes of S. cerevisiae. These genes are required for cytokinesis, and their products are present at the bud neck during cell division. We find that pnut is also required for cytokinesis: in pnut mutants, imaginal tissues fail to proliferate and instead develop clusters of large, multinucleate cells. Pnut protein is localized to the cleavage furrow of dividing cells during cytokinesis and to the intercellular bridge connecting postmitotic daughter cells. In addition to its role in cytokinesis, pnut displays genetic interactions with seven in absentia, a gene required for neuronal fate determination in the compound eye, suggesting that pnut may have pleiotropic functions. Our results suggest that this class of proteins is involved in aspects of cytokinesis that have been conserved between flies and yeast.