A member of the sugar transporter family, Stl1p is the glycerol/H+ symporter in Saccharomyces cerevisiae
A member of the sugar transporter family, Stl1p is the glycerol/H+ symporter in Saccharomyces cerevisiae
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DOI：
10.1091/mbc.e04-10-0884
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发表时间：
2005-04-01
影响因子：
3.3
通讯作者：
Brandt, A
中科院分区：
文献类型：
--
作者：
Ferreira, C;van Voorst, F;Brandt, A
甘油和其他多元醇被许多生物用作渗透保护剂。几种酵母菌和其他真菌可以通过质子运输来吸收甘油。为了鉴定与酿酒酵母主动摄取甘油有关的基因，我们筛选了一个缺失突变体库，该突变体库包含321个编码蛋白的基因，这些基因具有6个或6个以上预测的跨膜结构域，在甘油介质中生长受阻。STL1编码糖转运蛋白家族的一个成员，它的缺失消除了甘油的主动转运。在甘油转运蛋白发挥功能的条件下，Stl1p存在于酿酒酵母的质膜中。Stl1蛋白和主动的甘油运输都受到葡萄糖诱导的失活，遵循相同的模式。此外，当细胞受到渗透性休克时，Stl1蛋白和甘油同向转运体活性被强烈但短暂地诱导。STL1在裂殖酵母中异源表达，裂殖酵母不含自身活性的甘油运输系统。在S.pombe中，STL1以一种依赖于质子动力的方式赋予了在浓度梯度下摄取甘油的能力。我们认为酿酒酵母的甘油质子转运蛋白是由STL1编码的。
Glycerol and other polyols are used as osmoprotectants by many organisms. Several yeasts and other fungi can take up glycerol by proton symport. To identify genes involved in active glycerol uptake in Saccharomyces cerevisiae we screened a deletion mutant collection comprising 321 genes encoding proteins with 6 or more predicted transmembrane domains for impaired growth on glycerol medium. Deletion of STL1, which encodes a member of the sugar transporter family, eliminates active glycerol transport. Stl1p is present in the plasma membrane in S. cerevisiae during conditions where glycerol symport is functional. Both the Stl1 protein and the active glycerol transport are subject to glucose-induced inactivation, following identical patterns. Furthermore, the Stl1 protein and the glycerol symporter activity are strongly but transiently induced when cells are subjected to osmotic shock. STL1 was heterologously expressed in Schizosaccharomyces pombe, a yeast that does not contain its own active glycerol transport system. In S. pombe, STL1 conferred the ability to take up glycerol against a concentration gradient in a proton motive force-dependent manner. We conclude that the glycerol proton symporter in S. cerevisiae is encoded by STL1.