Response to stress in Drosophila is mediated by gender, age and stress paradigm.
Response to stress in Drosophila is mediated by gender, age and stress paradigm.
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DOI：
10.3109/10253890.2015.1017465
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Nieto-Romero AR
中科院分区：
文献类型：
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作者：
Neckameyer WS;Nieto-Romero AR
所有生物都必须保持平衡，以应对其环境中的内部和外部挑战。神经可塑性的改变（神经元群的改变、树突重塑和突触更新）是应激稳态反应的关键组成部分，与情感性障碍的发病密切相关。然而，根据压力的类型和环境，以及遗传背景、年龄和性别，人们对压力的感知是不同的；因此，个体对神经元稳态的维持一定会根据这些变量而有所不同。我们建立了果蝇作为模型来分析对压力的稳态反应。在控制环境条件下饲养的一种等基因野生型菌株，性成熟和性成熟的雌性和雄性暴露于四种可重复和高通量的可翻译应激源，以便对大量动物进行直接比较分析。这些动物在露天竞技场、明暗箱和强迫游泳试验中进行了评估，并对乙醇的镇静作用进行了敏感性评估。这些研究证实，在控制条件下以及暴露于不同压力源后，未成熟和成熟的雌性和雄性代表了不同的行为种群。因此，介导应激反应的神经基质必须根据大脑的激素状态差异表达。此外，在一种范式中，对给定压力源的适应性反应不能预测其他范式的结果。
All living organisms must maintain equilibrium in response to internal and external challenges within their environment. Changes in neural plasticity (alterations in neuronal populations, dendritic remodeling, and synaptic turnover) are critical components of the homeostatic response to stress, which has been strongly implicated in the onset of affective disorders. However, stress is differentially perceived depending on the type of stress and its context, as well as genetic background, age and sex; therefore, an individual’s maintenance of neuronal homeostasis must differ depending upon these variables. We established Drosophila as a model to analyze homeostatic responses to stress. Sexually immature and mature females and males from an isogenic wild-type strain raised under controlled environmental conditions were exposed to four reproducible and high-throughput translatable stressors to facilitate the analysis of a large number of animals for direct comparisons. These animals were assessed in an open-field arena, in a Light-Dark Box, and in a Forced Swim Test, as well as for sensitivity to the sedative effects of ethanol. These studies establish that immature and mature females and males represent behaviorally distinct populations under control conditions as well as after exposure to different stressors. Therefore, the neural substrates mediating the stress response must be differentially expressed depending upon the hormonal status of the brain. In addition, an adaptive response to a given stressor in one paradigm was not predictive for outcomes in other paradigms.