In vitro Cell Migration and Invasion Assays
In vitro Cell Migration and Invasion Assays
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DOI：
10.3791/51046
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发表时间：
2014-06-01
影响因子：
1.2
通讯作者：
Yang, Li V.
中科院分区：
文献类型：
--
作者：
Justus, Calvin R.;Leffler, Nancy;Yang, Li V.
迁移是活细胞的关键属性，对正常发育、免疫反应以及癌症转移和炎症等疾病过程至关重要。检测细胞迁移的方法对于癌症生物学、免疫学、血管生物学、细胞生物学和发育生物学等广泛的生物医学研究非常有用和重要。在这里，我们以肿瘤细胞的迁移和侵袭为例，描述了两种相关的检测方法，以说明常用的、容易获得的方法来测量这些过程。第一种方法是细胞培养伤口闭合试验，在融合的细胞单层上产生划痕。通过用常规倒置显微镜在几个时间间隔拍摄快照照片，可以量化伤口闭合和细胞迁移的速度。使用延时显微镜系统可以记录更详细的细胞迁移行为。本文描述的第二种方法是Transwell细胞迁移和侵袭试验，它测量细胞对化学吸引剂梯度的移动和侵袭能力。我们的目标是以一种高度可访问的方式描述这些方法，以便即使在基本的细胞生物学设置下，这些程序也可以在研究实验室成功执行。
Migration is a key property of live cells and critical for normal development, immune response, and disease processes such as cancer metastasis and inflammation. Methods to examine cell migration are very useful and important for a wide range of biomedical research such as cancer biology, immunology, vascular biology, cell biology and developmental biology. Here we use tumor cell migration and invasion as an example and describe two related assays to illustrate the commonly used, easily accessible methods to measure these processes. The first method is the cell culture wound closure assay in which a scratch is generated on a confluent cell monolayer. The speed of wound closure and cell migration can be quantified by taking snapshot pictures with a regular inverted microscope at several time intervals. More detailed cell migratory behavior can be documented using the time-lapse microscopy system. The second method described in this paper is the transwell cell migration and invasion assay that measures the capacity of cell motility and invasiveness toward a chemo-attractant gradient. It is our goal to describe these methods in a highly accessible manner so that the procedures can be successfully performed in research laboratories even just with basic cell biology setup.