Human Glomerular Basement Membrane: Altered Binding Characteristics following in Vitro Non‐enzymatic Glycosylation a
Human Glomerular Basement Membrane: Altered Binding Characteristics following in Vitro Non‐enzymatic Glycosylation a
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人肾小球基底膜：体外非酶糖基化后结合特性的改变
DOI：
10.1111/j.1749-6632.1986.tb54444.x
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发表时间：
1986
影响因子：
5.2
通讯作者：
D. Andreani
中科院分区：
文献类型：
--
作者：
M. Sensi;P. Tanzi;M. R. Bruno;M. Mancuso;D. Andreani
在过去的十年中，与糖尿病高血糖症相关的身体蛋白质的非酶糖基化过程变得非常重要。现在认为，由反应性糖基化终产物的形成诱导的结构和功能蛋白质改变部分涉及糖尿病性白内障、神经病和胶原蛋白改变的发展。肾脏是糖尿病血管并发症最易发生的器官之一，非酶糖基化也可能在其中发挥重要的致病作用。例如，众所周知的白蛋白、IgG、纤维蛋白和其他血浆蛋白沿着糖尿病肾脏的肾小球基底膜（GBM）的沉积可以通过由过度糖基化诱导的GBM胶原的改变的化学性质来介导。在患有糖尿病的动物3和人类4中发现的GBM糖基化增加的报告间接支持了这一假设。在这项研究中，我们研究了是否正常人GBM的反应性改变后，其增强体外非酶糖基化。
During the last decade, the process of non-enzymatic glycosylation of body proteins associated with the hyperglycemia of diabetes mellitus has grown very much in importance. It is now believed that the structural and functional protein alterations induced by the formation of reactive glycosylation end products are partly implicated in the development of diabetic cataracts, neuropathy, and collagen alterations.’ In the kidney, one of the organs most affected by diabetic vascular complications, nonenzymatic glycosylation could also play an important pathogenetic role. For example, the well known deposition of albumin, IgG, fibrin, and other plasma proteins along the glomerular basement membrane (GBM) of diabetic kidneys’ could be mediated via an altered chemistry of GBM collagen induced by excessive glycosylation. Indirect support for this hypothesis is given by the reports of increased GBM glycosylation found in animals3 and humans4 with diabetes. In this study we have investigated whether the reactivity of normal human GBM is altered after its enhanced in vitro non-enzymatic glycosylation.