Antioxidant effects of equol on bovine aortic endothelial cells
Antioxidant effects of equol on bovine aortic endothelial cells
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DOI：
10.1016/j.bbrc.2008.08.027
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发表时间：
2008-10-24
影响因子：
3.1
通讯作者：
Kim, Mee-Ran
中科院分区：
文献类型：
--
作者：
Chung, Jae-Eun;Kim, Sue Yeon;Kim, Mee-Ran
本研究旨在探讨雌马酚对H2 O2诱导的牛主动脉内皮细胞（bAECs）死亡的影响及其机制。MTT[3-（4，5-dimethylthiazol-2-yl）-2，5-diphenyl-tetrazolium bromide]法显示，对照组细胞存活率和存活率均显著下降，与H_2O_2刺激时间成正比（P < 0.05），而雌马酚预处理组细胞存活率和存活率下降无统计学意义。Hoechst 33342染色后，雌马酚预处理组中具有凋亡形态的细胞数量显著减少。雌马酚预处理可通过减少细胞内ROS的产生而有效抑制H2 O2诱导的细胞死亡（P < 0.05）。与未经雌马酚预处理的bAEC的表达相比，用雌马酚孵育bAEC在H2 O2暴露后增加了磷酸化p38 MAPK和Bcl-2的表达。此外，SB 203580抑制磷酸化p38 MAPK的表达，并增加凋亡细胞死亡。本研究证明雌马酚对暴露于H2 O2的bAEC具有显著的抗氧化作用。(C)2008年爱思唯尔公司All rights reserved.
This study is to examine the effects of equol on the H2O2-induced death of bovine aortic endothelial cells (bAECs) and the mechanism of its protective effects. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] assay showed that in the control group, cell survival Fate decreased significantly, each in proportion to the duration of the H2O2 Stimulation (P < 0.05), but, in the equol-pretreated group, such decrease was not statistically significant. After Hoechst 33342 staining, in the equol-pretreated group the number of cells with apoptotic morphology decreased significantly. Equol pretreatment effectively inhibited the H2O2-induced cell death by the reduction of intracellular ROS production (P < 0.05). Incubation of bAECs with equol increased the expression of phospho-p38 MAPK and Bcl-2 after the H2O2 exposure compared with their expression without the equol pretreatment. Furthermore, SB203580 inhibited phospho-p38 MAPK expression and increased apoptotic cell death. This Study proves equol has a significant antioxidant effect on the bAECs that were exposed to H2O2. (C) 2008 Elsevier Inc. All rights reserved.