Polyphosphate/poly-(R)-3-hydroxybutyrate) ion channels in cell membranes.
Polyphosphate/poly-(R)-3-hydroxybutyrate) ion channels in cell membranes.
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细胞膜中的聚磷酸盐/聚(R)-3-羟基丁酸)离子通道。
DOI：
10.1007/978-3-642-58444-2_8
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发表时间：
1999
影响因子：
--
通讯作者：
R. Reusch
中科院分区：
文献类型：
--
作者：
R. Reusch
由无机多磷酸盐（polyP）形成的最有趣的结构之一是它们与聚（R）-3-羟基丁酸酯（PHB）的复合物，所述复合物在脂质双层中形成离子通道。所有肽和蛋白质离子通道以及合成离子通道都是两亲性结构，具有非极性残基的外层和极性和带电残基的内层（Urry 1985; Christensen et al. 1988;中野et al. 1990; Sansom 1991; Kobuke et al. 1992; Epand 1993; Fyles et al. 1993）。这些属性由两种结构不同的均聚物聚P和聚羟基丁酸酯以协同方式提供。聚合阴离子聚P形成横跨双层的阳离子结合位点的阶梯，通过两亲性溶剂化聚酯PHB与疏水环境屏蔽。位于不同细菌质膜中的聚P和PHB的复合物（Reusch和Sadoff 1983; Reusch等1986，1987）是在生物细胞中发现的第一个非蛋白质的离子选择性通道。已确定其在平面脂质双层中形成电压激活的钙选择性通道的能力（Reusch et al. 1995; Das et al. 1997）。迄今为止，还没有直接证据表明其体内功能;然而，许多研究指出钙参与细菌的重要细胞功能，如趋化性（Matsushita et al. 1989; Tisa and Adler 1992）和细胞分裂（Chang 1986; Smith 1995; Norris 1996）。此外，有大量证据表明，聚P/PHB复合物可以作为钙泵和DNA通道。在这里，我们将讨论聚P和聚羟基丁酸酯的奇异分子特性，涉及到他们的作用，在离子转运，并考虑如何在协同作用，形成这些有趣的跨膜离子通道的两种聚合物。
Among the most intriguing structures formed by inorganic polyphosphates (polyP) are their complexes with poly-(R)-3-hydroxybutyrates) (PHB) that form ion channels in lipid bilayers. All peptide and protein ion channels, as well as synthetic ion channels, are amphiphilic structures with an outer coat of non-polar residues and a lining of polar and charged residues (Urry 1985; Christensen et al. 1988; Nakano et al. 1990; Sansom 1991; Kobuke et al. 1992; Epand 1993; Fyles et al. 1993). These attributes are provided in a cooperative fashion by the two structurally distinct homopolymers, polyP and PHB. The polymeric anion, polyP, forms a ladder of cation binding sites that stretches across the bilayer, shielded from the hydrophobic environment by the amphiphilic solvating polyester, PHB. Complexes of polyP and PHB, located in the plasma membranes of diverse bacteria (Reusch and Sadoff 1983; Reusch et al. 1986, 1987), are the first nonproteinaceous, ion-selective channels discovered in biological cells. Their capacity to form voltage-activated, calcium-selective channels in planar lipid bilayers has been established (Reusch et al. 1995; Das et al. 1997). As yet, there is no direct evidence of their in vivo functions; however, a number of studies point to calcium involvement in important cellular functions in bacteria, such as chemotaxis (Matsushita et al. 1989; Tisa and Adler 1992) and cell division (Chang 1986; Smith 1995; Norris 1996). In addition, there is substantial evidence that polyP/PHB complexes may serve as calcium pumps and DNA channels. Here we will discuss the singular molecular characteristics of polyP and PHB that relate to their roles in ion transport, and consider how the two polymers act in synergy to form these interesting transmembrane ion channels.
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DOI：
10.1016/s0021-9258(18)45243-x
发表时间：
1987-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Gangola;Barry P. RosenS
通讯作者：
P. Gangola;Barry P. RosenS
DOI：
10.1016/s0021-9258(19)38459-5
发表时间：
1990-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Ahn;A. Kornberg
通讯作者：
K. Ahn;A. Kornberg
从红细胞膜纯化的钙依赖性三磷酸腺苷酶催化循环中的部分反应。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kosk-Kosicka,D;Scaillet,S;Inesi,G
通讯作者：
Inesi,G
DOI：
10.1073/pnas.89.24.11804
发表时间：
1992
影响因子：
11.1
作者：
Tisa,LS;Adler,J
通讯作者：
Adler,J
DOI：
10.1073/pnas.85.14.5072
发表时间：
1988-07-01
影响因子：
11.1
作者：
CHRISTENSEN, B;FINK, J;MAUZERALL, D
通讯作者：
MAUZERALL, D