CD1d expression level in tumor cells is an important determinant for anti-tumor immunity by natural killer T cells
CD1d expression level in tumor cells is an important determinant for anti-tumor immunity by natural killer T cells
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DOI：
10.1080/10428190600682688
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发表时间：
2006-10-01
影响因子：
2.6
通讯作者：
Chiba, Shigeru
中科院分区：
文献类型：
--
作者：
Haraguchi, Kyoko;Takahashi, Tsuyoshi;Chiba, Shigeru
不变的自然杀伤T（iNKT）细胞被认为调节抗肿瘤免疫。据报道，人iNKT（即V α 24（+）NKT）细胞可识别靶细胞上的CD 1d，并在体外直接对靶细胞显示细胞毒性。然而，反复报道小鼠iNKT（即V α 14（+）NKT）细胞的抗肿瘤作用依赖于自然杀伤（NK）细胞通过干扰素-γ的活性，没有直接细胞毒性的证据。在本研究中，我们报告了V α 24（+）NKT细胞对EL-4小鼠淋巴母细胞性淋巴瘤细胞的体外细胞溶解和这些细胞的体内根除都依赖于肿瘤细胞表面CD 1d表达水平。这些观察结果可能表明，iNKT细胞对肿瘤细胞的直接细胞毒性在人类和小鼠中是常见的，并且CD 1d的高表达水平可能是肿瘤是否是iNKT细胞的良好靶点的预测因子。
Invariant natural killer T (iNKT) cells are thought to regulate anti-tumor immunity. Human iNKT (i.e. V alpha 24(+) NKT) cells have been reported to recognize CD1d on target cells and show cytotoxicity directly on the target cells in vitro. However, the anti-tumor effect of mouse iNKT (i.e. V alpha 14(+) NKT) cells has been repeatedly reported to be dependent on the activity of natural killer (NK) cells via interferon-gamma, with no evidence of direct cytotoxicity. In the present study, we report that in vitro cytolysis of EL-4 mouse lymphoblastic lymphoma cells by V alpha 24(+) NKT cells and in vivo eradication of these cells are both dependent on the level of CD1d expression on the tumor cell surface. These observations possibly suggest that direct cytotoxicity of tumor cells by iNKT cells is common to both humans and mice, and that the high expression level of CD1d may be a predictor whether the tumor is a good target of iNKT cells.