In vivo expression of mRNAs encoding hypoxia-inducible factor 1
In vivo expression of mRNAs encoding hypoxia-inducible factor 1
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DOI：
10.1006/bbrc.1996.1199
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发表时间：
1996-08-14
影响因子：
3.1
通讯作者：
Semenza, GL
中科院分区：
文献类型：
--
作者：
Wiener, CM;Booth, G;Semenza, GL
缺氧诱导因子 1 (HIF-1) 是一种异二聚体碱性螺旋-环-螺旋转录因子，可调节基因，其产物在维持 O-2 稳态中发挥关键作用。我们之前已经证明，当哺乳动物组织培养细胞处于缺氧状态时，HIF-1 mRNA、蛋白质和 DNA 结合活性会被诱导。在本文中，我们报告了体内 HIF-1 mRNA 表达的分析。我们在所有检测的人类、大鼠和小鼠器官中证明了 HIF-1 α 和 HIF-1 β (ARNT) mRNA 的表达，并首次表明当大鼠或小鼠暴露于降低的环境 O-2 浓度 30 至 60 分钟时，大脑、肾脏和肺中会诱导 HIF-1 mRNA 表达。 HIF-1 α 和 HIF-1 β (ARNT) mRNA 在体内的普遍表达与 HIF-1 在协调缺氧适应性转录反应中的作用一致。 (C) 1996 学术出版社
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric basic helix-loop-helix transcription factor that regulates genes whose products play key roles in maintaining O-2 homeostasis. We have previously demonstrated that HIF-1 mRNA, protein, and DNA-binding activity are induced when mammalian tissue culture cells are subjected to hypoxia. In this paper, we report our analysis of HIF-1 mRNA expression in vivo. We demonstrate expression of HIF-1 alpha and HIF-1 beta (ARNT) mRNA in all human, rat, and mouse organs assayed and show for the first time that HIF-1 mRNA expression was induced in brain, kidney, and lung when rats or mice were exposed to reduced ambient O-2 concentrations for 30 to 60 min. The ubiquitous in vivo expression of HIF-1 alpha and HIF-1 beta (ARNT) mRNA is consistent with the proposed role of HIF-1 in coordinating adaptive transcriptional responses to hypoxia. (C) 1996 Academic Press, Inc.