Movement of yeast cortical actin cytoskeleton visualized in vivo
Movement of yeast cortical actin cytoskeleton visualized in vivo
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DOI：
10.1073/pnas.93.9.3886
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发表时间：
1996-04-30
影响因子：
11.1
通讯作者：
Botstein, D
中科院分区：
文献类型：
--
作者：
Doyle, T;Botstein, D
出芽酵母（Saccharomyces cerevisiae）中绿色荧光蛋白（GFP）与细胞骨架蛋白Act1p（肌动蛋白）、Sac6p（酵母纤维蛋白同源物）和Abp1p之间的融合蛋白定位于皮层肌动蛋白斑块。肌动蛋白融合不能作为酵母中肌动蛋白的唯一来源，但肌动蛋白结合蛋白Abp1p和Sac6p之间的融合完全补充了与其基因缺失相关的表型。在体内的直接观察显示肌动蛋白皮质斑块有移动。肌动蛋白贴片的运动受限于生长细胞中贴片的不对称分布，当添加叠氮化钠等代谢抑制剂时，这种运动大大减少。融合蛋白标记斑块在酵母细胞周期和交配过程中呈正态分布。在体内的观察也使得在产孢过程中肌动蛋白斑块的可视化成为可能。
Fusion proteins between the green fluorescent protein (GFP) and the cytoskeleton proteins Act1p (actin), Sac6p (yeast fimbrin homolog), and Abp1p in budding yeast (Saccharomyces cerevisiae) localize to the cortical actin patches. The actin fusions could not function as the sole actin source in yeast, but fusions between the actin-binding proteins Abp1p and Sac6p complement fully the phenotypes associated with their gene deletions. Direct observation in vivo reveals that the actin cortical patches move. Movement of actin patches is constrained to the asymmetric distribution of the patches in growing cells, and this movement is greatly reduced when metabolic inhibitors such as sodium azide are added. Fusion protein-labeled patches are normally distributed during the yeast cell cycle and during mating. In vivo observation made possible the visualization of actin patches during sporulation as well.