Near‐Infrared Light Triggered‐Release in Deep Brain Regions Using Ultra‐photosensitive Nanovesicles
Near‐Infrared Light Triggered‐Release in Deep Brain Regions Using Ultra‐photosensitive Nanovesicles
复制标题
使用超光敏纳米囊泡在大脑深部区域触发近红外光释放
DOI：
10.1002/ange.201915296
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发表时间：
2020
影响因子：
--
通讯作者：
Zasadzinski, Joseph A.
中科院分区：
文献类型：
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作者：
Xiong, Hejian;Li, Xiuying;Kang, Peiyuan;Perish, John;Neuhaus, Frederik;Ploski, Jonathan E.;Kroener, Sven;Ogunyankin, Maria O.;Shin, Jeong Eun;Zasadzinski, Joseph A.
利用光对生物系统进行远程和微创调制已经改变了现代生物学和神经科学。然而，光的吸收和散射显著地阻止了对脑深部区域的渗透。在本文中，我们描述了金涂层的机械响应纳米囊泡的用途，该纳米囊泡由人工磷脂Rad-PC-Rad制成的脂质体组成，作为将生物活性分子递送到脑组织中的工具。近红外皮秒激光脉冲激活了纳米囊泡表面的金涂层，产生纳米机械应力，并导致在亚秒内几乎完全释放囊泡货物。与天然磷脂脂质体相比，光释放可能在低40倍的激光能量下进行。这种高光敏性使得分子的光释放能够在小鼠大脑中达到4 mm的深度。 这个有前途的工具提供了一个多功能平台，可以光学释放功能分子来调节大脑回路。
Remote and minimally‐invasive modulation of biological systems with light has transformed modern biology and neuroscience. However, light absorption and scattering significantly prevents penetration to deep brain regions. Herein, we describe the use of gold‐coated mechanoresponsive nanovesicles, which consist of liposomes made from the artificial phospholipid Rad‐PC‐Rad as a tool for the delivery of bioactive molecules into brain tissue. Near‐infrared picosecond laser pulses activated the gold‐coating on the surface of nanovesicles, creating nanomechanical stress and leading to near‐complete vesicle cargo release in sub‐seconds. Compared to natural phospholipid liposomes, the photo‐release was possible at 40 times lower laser energy. This high photosensitivity enables photorelease of molecules down to a depth of 4 mm in mouse brain. This promising tool provides a versatile platform to optically release functional molecules to modulate brain circuits.