Poly(β-L-malic acid) production by diverse phylogenetic clades of Aureobasidium pullulans
Poly(β-L-malic acid) production by diverse phylogenetic clades of Aureobasidium pullulans
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DOI：
10.1007/s10295-011-1007-7
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发表时间：
2012-01-01
影响因子：
3.4
通讯作者：
Leathers, Timothy D.
中科院分区：
文献类型：
--
作者：
Manitchotpisit, Pennapa;Skory, Christopher D.;Leathers, Timothy D.
聚（β - L - 苹果酸）（PMA）是一种天然生物聚酯，具有药物应用及其他潜在用途。在本研究中，我们检测了代表遗传多样性系统发育分支的56株出芽短梗霉（Aureobasidium pullulans）的PMA产量。其中36株是从冰岛和泰国的不同地点分离得到的。来自冰岛的所有菌株都属于一个新认定的第13分支，而来自泰国的菌株分布在其他8个分支中，包括一个新的第14分支。将这些分离株中的30株以及26株先前描述过的菌株，在含有5%葡萄糖的培养基中检测PMA产量。大多数菌株每升至少产生4克PMA，第9、11和13分支中的一些菌株每升可产生9 - 11克PMA。这些菌株还产生普鲁兰多糖和重油，但通过乙醇差异沉淀法分离得到的PMA纯度高达72%，普鲁兰多糖污染不超过12%。出芽短梗霉产生的PMA分子量在5.1到7.9 kDa之间。结果表明，出芽短梗霉的某些遗传群体在PMA生产方面很有前景。
Poly(beta-L-malic acid) (PMA) is a natural biopolyester that has pharmaceutical applications and other potential uses. In this study, we examined PMA production by 56 strains of the fungus Aureobasidium pullulans representing genetically diverse phylogenetic clades. Thirty-six strains were isolated from various locations in Iceland and Thailand. All strains from Iceland belonged to a newly recognized clade 13, while strains from Thailand were distributed among 8 other clades, including a novel clade 14. Thirty of these isolates, along with 26 previously described strains, were examined for PMA production in medium containing 5% glucose. Most strains produced at least 4 g PMA/L, and several strains in clades 9, 11, and 13 made 9-11 g PMA/L. Strains also produced both pullulan and heavy oil, but PMA isolated by differential precipitation in ethanol exhibited up to 72% purity with no more than 12% contamination by pullulan. The molecular weight of PMA from A. pullulans ranged from 5.1 to 7.9 kDa. Results indicate that certain genetic groups of A. pullulans are promising for the production of PMA.