A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus
A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus
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DOI：
10.1002/1097-4636(20001215)52:4
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发表时间：
2000-12-15
期刊：
影响因子：
--
通讯作者：
Kim, JO
中科院分区：
文献类型：
--
作者：
Feng, QL;Wu, J;Kim, JO
为探讨银离子对微生物的抑制作用机理，以革兰氏阴性大肠杆菌（E。大肠杆菌和革兰氏阳性金黄色葡萄球菌（S.金黄色葡萄球菌），用硝酸银处理，并研究了结合电子显微镜和X射线微分析。在大肠杆菌和沙门氏菌中也发生了类似的形态学变化。金黄色葡萄球菌细胞。细胞质膜从细胞壁上脱落。在细胞中心出现了一个显著的电子光区域，其中含有浓缩的脱氧核糖核酸（DNA）分子。在细胞壁周围或细胞内部有许多电子致密的小颗粒。X-射线微区分析表明，银离子处理后，电子致密颗粒和细胞质中均存在银和硫元素，提示银的抗菌机理是DNA失去复制能力，蛋白质失活。S. aureus与E. coli推荐了S.金黄色葡萄球菌对抗Ag+离子的抑制作用。(C)John Wiley & Sons，Inc.
To investigate the mechanism of inhibition of silver ions on microorganisms, two strains of bacteria, namely Gram-negative Escherichia coli (E. coli and Gram-positive Staphylococcus aureus (S. aureus), were treated with AgNO3 and studied using combined electron microscopy and X-ray microanalysis. Similar morphological changes occurred in both E.coli and S. aureus cells after Ag+ treatment. The cytoplasm membrane detached from the cell wall. A remarkable electron-light region appeared in the center of the cells, which contained condensed deoxyribonucleic acid (DNA) molecules. There are many small electron-dense granules either surrounding the cell wall or depositing inside the cells. The existence of elements of silver and sulfur in the electron-dense granules and cytoplasm detected by X-ray microanalysis suggested the antibacterial mechanism of silver: DNA lost its replication ability and the protein became inactivated after Ag+ treatment. The slighter morphological changes of S. aureus compared with E. coli recommended a defense system of S. aureus against the inhibitory effects of Ag+ ions. (C) 2000 John Wiley & Sons, Inc.