The Rid family member RutC of Escherichia coli is a 3-aminoacrylate deaminase.
The Rid family member RutC of Escherichia coli is a 3-aminoacrylate deaminase.
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DOI：
10.1016/j.jbc.2021.100651
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Downs DM
中科院分区：
文献类型：
--
作者：
Buckner BA;Lato AM;Campagna SR;Downs DM
Rid蛋白家族（PF 14588，IPR 006175）分为九个亚家族，其中只有RidA亚家族已被生物化学表征。RutC是一个亚家族的创始成员，在嘧啶利用（rut）操纵子中编码，该操纵子编码允许大肠杆菌使用尿嘧啶作为唯一氮源的途径。本文报道的结果表明，RutC具有3-氨基丙烯酸酯脱氨酶活性，并促进以前推测在体内自发发生的反应之一。RutC与几种烯胺-亚胺底物具有活性，与Rid超家族的典型成员肠道沙门氏菌RidA在底物特异性方面表现出相似性和差异。在标准实验室条件下，缺乏RutC的Rut途径从尿嘧啶产生足够的氮用于大肠杆菌的生长。这些结果支持了Rut途径的修正模型，并提供了Rid蛋白可能调节代谢适应性而不是催化基本功能的证据。
The Rid protein family (PF14588, IPR006175) is divided into nine subfamilies, of which only the RidA subfamily has been characterized biochemically. RutC, the founding member of one subfamily, is encoded in the pyrimidine utilization (rut) operon that encodes a pathway that allows Escherichia coli to use uracil as a sole nitrogen source. Results reported herein demonstrate that RutC has 3-aminoacrylate deaminase activity and facilitates one of the reactions previously presumed to occur spontaneously in vivo. RutC was active with several enamine–imine substrates, showing similarities and differences in substrate specificity with the canonical member of the Rid superfamily, Salmonella enterica RidA. Under standard laboratory conditions, a Rut pathway lacking RutC generates sufficient nitrogen from uracil for growth of E. coli. These results support a revised model of the Rut pathway and provide evidence that Rid proteins may modulate metabolic fitness, rather than catalyzing essential functions.
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