Mutagenesis of the mouse germline using retroviruses.
Mutagenesis of the mouse germline using retroviruses.
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使用逆转录病毒对小鼠种系进行诱变。
DOI：
10.1007/978-3-642-76524-7_2
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发表时间：
1991
影响因子：
--
通讯作者：
Copeland,NG
中科院分区：
文献类型：
--
作者：
Lock,LF;Jenkins,NA;Copeland,NG
传统上，小鼠突变被认为是野生或实验室饲养的小鼠中自发发生的突变，或放射或化学处理小鼠中的诱导突变（GREEN 1981）。尽管从这些突变的研究中已经学到了很多东西，但对突变基因的分子分析往往因缺乏对它们的分子接触而受到阻碍。为了避免这个问题，转座元件可以用作插入诱变剂。突变基因内或附近存在的转座元件充当分子标签，有助于克隆感兴趣的突变基因。这种方法已在多种生物体中取得了巨大成功，最著名的是果蝇（Rubin1988）。
Traditionally, mutations in the mouse have been identified as spontaneously occurring mutations in feral or laboratory-maintained mice or as induced mutations in radiation-or chemical-treated mice (GREEN 1981). Although much has been learned from the study of these mutations, molecular analysis of the mutated genes has often been hampered by the lack of molecular access to them. To circumvent this problem, transposable elements can be used as insertional mutagens. The presence of a transposable element within or near the mutant gene acts as a molecular tag which aids in cloning the mutated gene of interest. This approach has been used with great success in several organisms, most notablyDrosophila melariogaster(Rubin1988).