New Approaches for Genome Assembly and Scaffolding
New Approaches for Genome Assembly and Scaffolding
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DOI：
10.1146/annurev-animal-020518-115344
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发表时间：
2019-01-01
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影响因子：
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通讯作者：
Green, Richard E.
中科院分区：
文献类型：
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作者：
Rice, Edward S.;Green, Richard E.
负担得起的高通量DNA测序在过去十年中加快了基因组组装的步伐。然而，来自高通量、短读段测序的基因组组装通常不像第一代基因组组装那样连续。尽管早期的基因组组装项目经常得到克隆图谱或其他映射数据的帮助，但目前的许多组装项目放弃了这些支架数据，只将基因组组装成更小的片段。最近，新技术的发明使得染色体规模的组装比传统方法成本更低，速度更快。在这里，我们概述了染色体规模组装的问题和解决这个问题的传统方法。然后，我们回顾了染色体规模组装的新技术和最近的基因组计划，使用这些技术以低成本创建高度连续的基因组组装。
Affordable, high-throughput DNA sequencing has accelerated the pace of genome assembly over the past decade. Genome assemblies from highthroughput, short-read sequencing, however, are often not as contiguous as the first generation of genome assemblies. Whereas early genome assembly projects were often aided by clone maps or other mapping data, many current assembly projects forego these scaffolding data and only assemble genomes into smaller segments. Recently, new technologies have been invented that allow chromosome-scale assembly at a lower cost and faster speed than traditional methods. Here, we give an overview of the problem of chromosome-scale assembly and traditional methods for tackling this problem. We then review new technologies for chromosome-scale assembly and recent genome projects that used these technologies to create highly contiguous genome assemblies at low cost.