The Clostridium difficile spo0A Gene Is a Persistence and Transmission Factor
The Clostridium difficile spo0A Gene Is a Persistence and Transmission Factor
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DOI：
10.1128/iai.00147-12
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发表时间：
2012-08-01
影响因子：
3.1
通讯作者：
Lawley, Trevor D.
中科院分区：
文献类型：
--
作者：
Deakin, Laura J.;Clare, Simon;Lawley, Trevor D.
艰难梭菌是慢性抗生素相关性腹泻的主要原因，也是一种重要的卫生保健相关病原体，可形成高度耐药和传染性的孢子。 Spo0A 是一种高度保守的转录调节因子，在芽孢杆菌和梭菌属物种的孢子形成启动中发挥关键作用。在这里，我们使用小鼠模型来研究艰难梭菌 spo0A 基因在感染和传播过程中的作用。我们证明艰难梭菌 spo0A 突变衍生物可以引起肠道疾病，但无法在小鼠体内持续存在并在小鼠之间有效传播。因此，艰难梭菌 Spo0A 蛋白在持续感染中发挥着关键作用，包括小鼠中的复发和宿主间传播。
Clostridium difficile is a major cause of chronic antibiotic-associated diarrhea and a significant health care-associated pathogen that forms highly resistant and infectious spores. Spo0A is a highly conserved transcriptional regulator that plays a key role in initiating sporulation in Bacillus and Clostridium species. Here, we use a murine model to study the role of the C. difficile spo0A gene during infection and transmission. We demonstrate that C. difficile spo0A mutant derivatives can cause intestinal disease but are unable to persist within and effectively transmit between mice. Thus, the C. difficile Spo0A protein plays a key role in persistent infection, including recurrence and host-to-host transmission in mice.