Ovarian follicular function is not altered by SARS-CoV-2 infection or BNTI62b2 mRNA COVID-19 vaccination
Ovarian follicular function is not altered by SARS-CoV-2 infection or BNTI62b2 mRNA COVID-19 vaccination
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DOI：
10.1093/humrep/deab182
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发表时间：
2021-08-07
影响因子：
6.1
通讯作者：
Klement, A. Hersko
中科院分区：
文献类型：
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作者：
Bentov, Y.;Beharier, O.;Klement, A. Hersko
研究问题：对冠状病毒病2019年(新冠肺炎)感染或BNTI62132mRNA疫苗的免疫反应是否涉及卵巢卵泡，以及它是否影响其功能？摘要回答：我们能够在感染和接种了新冠肺炎的体外受精患者的卵泡液中证实抗SARS-CoV-2免疫球蛋白，没有证据表明卵泡功能受损。目前还没有研究数据。STUDY设计，大小，持续时间：这是一项队列研究，包括32名连续感染新冠肺炎的试管受精患者，无论是接种疫苗还是未暴露的，2021年2月1日/3月10日在一家以大学医院为基础的体外受精诊所完成。对象、地点、方法：连续选取一组接受取卵手术的女性患者，随机分为三组：新冠肺炎恢复组(n=9)；接种疫苗(n=9)；未感染、未接种疫苗的对照组(n=14)。检测血清和卵泡灌洗液中抗柯萨奇病毒免疫球蛋白、雌激素、孕酮和硫酸乙酰肝素蛋白多糖2浓度，以及抽吸卵母细胞的数量和成熟度以及雌孕激素的触发天数。主要观察指标是卵泡功能，包括类固醇激素生成、卵泡对促黄体生成激素/人绒毛膜促性腺激素触发的反应和卵母细胞质量生物标志物。主要结果和机会的作用：新冠肺炎和疫苗都能在卵泡生长因子中检测到抗冠状病毒免疫球蛋白抗体，抗体水平与免疫球蛋白血清浓度成正比。三组之间在卵巢卵泡质量的替代参数中没有发现任何差异。限制，谨慎的理由：这是一项小型研究，包括生育和不育人群，其结论应该得到更大规模研究的支持和验证。研究结果的广泛意义：这是第一项研究SARS-CoV-2感染和接种CoVID-I9疫苗对卵巢功能的影响，这些早期研究结果表明，没有可测量的有害影响卵巢卵泡的功能。
STUDY QUESTION: Does the immune response to coronavirus disease 2019 (COVID- 19) infection or the BNTI62132 mRNA vaccine involve the ovarian follicle, and does it affect its function?SUMMARY ANSWER: We were able to demonstrate anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG in follicular fluid (FF) from both infected and vaccinated IVF patients, with no evidence for compromised follicular function.WHAT IS KNOWN ALREADY: No research data are available yet.STUDY DESIGN, SIZE, DURATION: This is a cohort study, composed of 32 consecutive IVF patients, either infected with COVID-19, vaccinated or non-exposed, conducted between I February and 10 March 2021 in a single university hospital-based IVF clinic.PARTICIPANTS/MATERIALS, SETTING, METHODS: A consecutive sample of female consenting patients undergoing oocyte retrieval was recruited and assigned to one of the three study groups: recovering from confirmed COVID-19 (n = 9); vaccinated (n = 9); and uninfected, non-vaccinated controls (n = 14). Serum and FF samples were taken and analyzed for anti-COVID IgG as well as estrogen, progesterone and heparan sulfate proteoglycan 2 concentration, as well as the number and maturity of aspirated oocytes and day of trigger estrogen and progesterone measurements. Main outcome measures were follicular function, including steroidogenesis, follicular response to the LH/hCG trigger, and oocyte quality biomarkers.MAIN RESULTS AND THE ROLE OF CHANCE: Both COVID- 19 and the vaccine elicited anti-COVID IgG antibodies that were detected in the FF at levels proportional to the IgG serum concentration. No differences between the three groups were detected in any of the surrogate parameters for ovarian follicle quality.LIMITATIONS, REASONS FOR CAUTION: This is a small study, comprising a mixed fertile and infertile population, and its conclusions should be supported and validated by larger studies.WIDER IMPLICATIONS OF THE FINDINGS: This is the first study to examine the impact of SARS-Cov-2 infection and COVID-I 9 vaccination on ovarian function and these early findings suggest no measurable detrimental effect on function of the ovarian follicle.