Mechanisms of cetyltrimethyl ammonium chloride-induced toxicity to photosystem II oxygen evolution complex of Chlorella vulgaris F1068
Mechanisms of cetyltrimethyl ammonium chloride-induced toxicity to photosystem II oxygen evolution complex of Chlorella vulgaris F1068
复制标题
十六烷基三甲基氯化铵对小球藻 F1068 光系统 II 析氧复合体的毒性机制
DOI：
10.1016/j.jhazmat.2019.121063
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发表时间：
2020-02-05
影响因子：
13.6
通讯作者：
Ge, Fei
中科院分区：
文献类型：
--
作者：
Liu, Na;Zhang, Han;Ge, Fei
微藻光合作用对水环境中共存污染物敏感，从而对藻类生长和营养盐吸收产生不利影响。研究了十六烷基三甲基氯化铵（CTAC）对绿色小球藻F1068（C. vulgaris F1068）。结果表明，CTAC降低了藻类的生长速率和营养盐去除效率，并削弱了光合性能。同时，CTAC胁迫下的放氧复合物（OEC）效率和放氧速率也显著下降，分别为对照的90.48%和58.48%。原子力显微镜（AFM）检测到CTAC对PSII-OEC形貌和结构的破坏。此外，蛋白质组学分析表明，41%的蛋白质在叶绿体类囊体膜上，在光合作用中发挥作用。CTAC显著抑制了参与电子传递的放氧增强子蛋白2（OEE 2或PsbP）的活性，在0.6mg/L CTAC存在下，OEE 2或PsbP的活性下调了15.14倍。这些结果表明，CTAC的光合抑制主要发生在PSII-OEC。该研究为评价表面活性剂对微藻的环境生物影响提供了一个新的视角。
Microalgae photosynthesis is sensitive to coexisted contaminates in aquatic environment, thereby causes adverse effect on algal growth and nutrients uptake. Here, we investigated the photosynthetic toxicity mechanism of cetyltrimethyl ammonium chloride (CTAC)-induced on a green microalga Chlorella vulgaris F1068 (C. vulgaris F1068). Results showed that CTAC reduced the algal growth rate, nutrients removal efficiency and weakened the photosynthetic performance. Meanwhile, the efficiency of oxygen evolution complex (OEC) and oxygen evolvement rates stressed by CTAC were significantly declined to 90.48% and 58.48% of the control (without CTAC), respectively. In addition, atomic force microscopy (AFM) detected the damage of PSII-OEC morphology and structure by CTAC. Furthermore, proteomic analysis showed that 41% of proteins were in the chloroplast thylakoid membranes which function in photosynthesis. The activity of oxygen-evolving enhancer protein 2 (OEE2 or PsbP) involved in electron transfer was significantly inhibited by CTAC, which down-regulated 15.14-fold in the presence of 0.6 mg/L CTAC. These results indicated that photosynthetic inhibition of CTAC mainly occurred in the PSII-OEC. This study provided a new perspective of the photosynthetic response in evaluation of environmental bioimpacts of surfactants on microalgae.