Interfacial catalysis by phospholipase A2: monomeric enzyme is fully catalytically active at the bilayer interface.
Interfacial catalysis by phospholipase A2: monomeric enzyme is fully catalytically active at the bilayer interface.
复制标题
磷脂酶 A2 的界面催化：单体酶在双层界面具有完全催化活性。
DOI：
10.1021/bi00243a038
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发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Verheij,HM
中科院分区：
文献类型：
--
作者：
Jain,MK;Ranadive,G;Yu,BZ;Verheij,HM
材料和方法本研究中使用的所有试剂和缓冲液均为分析纯。 7V-甲基靛红酸酐购自Mo-1 缩写：AMPA，猪胰PLA2，其中所有赖氨酸的末端氨基已被脒化； An87-PLA2，猪胰PLA2在87位赖氨酸上用IV-甲基靛红酸酐共价修饰； DMPM，1，2-二肉豆蔻酰基-甘油-3-磷酸甲醇； DMPC，l，2-二肉豆蔻酰基-甘油-3-磷酸胆碱； DTPM，1, 2-双十四烷基甘油-3-磷酸甲醇； DTPC，1, 2-二十四烷基-甘油-3-磷酸胆碱； HDNS，丹磺酰化十六烷基磷酸乙醇胺； Me-PLA2，猪胰腺 PLA2 在 His-48 处被 4-硝基苯磺酸甲酯甲基化； N „每个酶分子水解的底物分子数；NOB，4-硝基-3-辛酰氧基苯甲酸酯；NOB-PLA2，从 NOB 转移辛酰基的猪胰腺 PLA2；Oct-PLA2，用 1-溴-2-辛酮在 His-48 处修饰的猪胰腺 PLA2；Palm-110 PLA2，猪胰PLA2，在赖氨酸110处具有棕榈酰基； PLA2，磷脂酶A2。
Materials and Methods All reagents and buffers used in this study were analytical grade. 7V-Methylisatoic anhydride was purchased from Mo-1 Abbreviations: AMPA, pig pancreatic PLA2 in which terminal amino groups of all lysines have been amidinated; An87-PLA2, pig pancreatic PLA2 covalently modified at lysine87 with IV-methylisatoic anhydride; DMPM, l, 2-dimyristoyl-in-glycero-3-phosphomethanol; DMPC, l, 2-dimyristoyI-jvi-giycero-3-phosphocholine; DTPM, 1, 2-ditetradecyl-in-glycero-3-phosphomethanol; DTPC, 1, 2-ditetradecyl-rnglycero-3-phosphocholine; HDNS, dansylated hexadecylphospho-ethanolamine; Me-PLA2, pig pancreatic PLA2 methylated at His-48 by methyl-4-nitrobenzene sulfonate; N „the number of substrate molecules hydrolyzed per enzyme molecule; NOB, 4-nitro-3-octanoyloxy benzoate; NOB-PLA2, pig pancreaticPLA2 to which an octanoyl group has been transferred from NOB; Oct-PLA2, pig pancreatic PLA2 modified at His-48 with 1-bromo-2-octanone; Palm-110 PLA2, pig pancreaticPLA2 with a palmitoyl group at lysine 110; PLA2, phospholipaseA2.