Cell type boundaries organize plant development.
Cell type boundaries organize plant development.
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DOI：
10.7554/elife.27421
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发表时间：
2017-09-12
期刊：
影响因子：
7.7
通讯作者：
Heisler MG
中科院分区：
文献类型：
--
作者：
Caggiano MP;Yu X;Bhatia N;Larsson A;Ram H;Ohno CK;Sappl P;Meyerowitz EM;Jönsson H;Heisler MG
在植物中，叶的背腹边界定义了一个对称轴，它穿过器官的中心，分隔顶部(背部)和底部(腹部)组织。虽然这一边界的位置对叶片的形态发生至关重要，但该边界是如何建立的，以及它是如何影响发育的仍不清楚。利用实时成像和扰动实验，我们发现HD-ZIPIII和Kan基因在地上部的表达预先决定了叶片的方向、形态和位置，从而形成了背腹基因通过在其表达区域之间定位生长素反应来协调植物发育的模型。然而，我们也发现生长素水平通过在枝条外围空间组织HD-ZIPIII和Kan的表达来反馈背腹模式。通过证明生长素对这些基因的调节也控制了它们对创伤的反应，我们的结果也为创伤对叶片背腹面的影响提供了一个简明的解释。
In plants the dorsoventral boundary of leaves defines an axis of symmetry through the centre of the organ separating the top (dorsal) and bottom (ventral) tissues. Although the positioning of this boundary is critical for leaf morphogenesis, how the boundary is established and how it influences development remains unclear. Using live-imaging and perturbation experiments we show that leaf orientation, morphology and position are pre-patterned by HD-ZIPIII and KAN gene expression in the shoot, leading to a model in which dorsoventral genes coordinate to regulate plant development by localizing auxin response between their expression domains. However we also find that auxin levels feedback on dorsoventral patterning by spatially organizing HD-ZIPIII and KAN expression in the shoot periphery. By demonstrating that the regulation of these genes by auxin also governs their response to wounds, our results also provide a parsimonious explanation for the influence of wounds on leaf dorsoventrality.