Determination and pharmacokinetic analysis of salvianolic acid B in rat blood and bile by microdialysis and liquid chromatography
Determination and pharmacokinetic analysis of salvianolic acid B in rat blood and bile by microdialysis and liquid chromatography
复制标题
DOI：
10.1016/j.chroma.2005.01.096
复制
发表时间：
2005-09-23
影响因子：
4.1
通讯作者：
Tsai, TH
中科院分区：
文献类型：
--
作者：
Chen, YF;Jaw, I;Tsai, TH
丹酚酸B是从丹参中分离得到的一种草药成分。建立了一种结合高效液相色谱的体内微透析取样方法，用于大鼠血液和胆汁中蛋白不结合丹酚酸B的连续监测。将微透析探针插入大鼠颈静脉/右心房及胆管，经股静脉给药100 mg/kg丹酚酸B。收集透析液并直接注入液相色谱系统。丹酚酸B采用微孔反相ODS 5 μ m（150朗姆酒x 1毫米内径）柱洗脱。用液相色谱系统在10 min内完成丹酚酸B的等密度洗脱。色谱流动相为乙腈-甲醇- 20mm磷酸钠（pH 3.5） （10:30:60, v/v/v），含0.1 mM 1-辛烷磺酸，流速0.05 ml/min。紫外检测器波长设置为290 nm。血和胆汁透析液中的丹酚酸B可用所述的液相色谱条件充分测定，尽管空白胆汁模式更为复杂。丹酚酸B在大鼠血液和胆汁透析液中的滞留时间为7.2 min。丹酚酸B的峰面积在0.1 ~ 50 μ g/ml范围内呈线性关系（r(2) bb0 0.995）。大鼠血液和胆汁中丹酚酸B微透析探针的体内回收率平均分别为22 +/- 2%和41 +/- 1%。本研究表明丹酚酸B通过肝胆排泄。(c) 2005 Elsevier B.V.版权所有
Salvianolic acid B is an herbal ingredient isolated from Salvia miltiorrhiza. An in vivo microdialysis sampling method coupled to high-performance liquid chromatography has been developed for continuous monitoring of protein-unbound salvianolic acid B in rat blood and bile. Microdialysis probes were inserted into the jugular vein/right atrium and bile duct of Sprague-Dawley rats, and a dose of 100 mg/kg salvianolic acid B was then administered via the femoral vein. Dialysates were collected and directly injected into a liquid chromatographic system. Salvianolic acid B was eluted using a microbore reversed-phase ODS 5 mu m (150 rum x I mm I.D.) column. Isocratic elution of salvianolic acid B was achieved within 10 min using the liquid chromatographic system. The chromatographic mobile phase consisted of acetonitrile-methanol-20mM monosodium phosphoric acid (pH 3.5) (10:30:60, v/v/v) containing 0.1 mM 1-octanesulfonic acid with 0.05 ml/min. The wavelength of the UV detector was set at 290 nm. Salvianolic acid B in both blood and bile dialysates was adequately determined using the liquid chromatographic conditions described, although the blank bile pattern was more complex. The retention times of salvianolic acid B in rat blood and bile dialysates were found to be 7.2 min. Peak-areas of salvianolic acid B were linear (r(2) > 0.995) over a concentration range of 0.1-50 mu g/ml. In vivo recoveries of microdialysis probes of salvianolic acid B in rat blood and bile averaged 22 +/- 2% and 41 +/- 1%, respectively. This study indicates that salvianolic acid B undergoes hepatobiliary excretion. (c) 2005 Elsevier B.V. All rights reserved.