Ethanol neuro-behavioural teratogenesis in the guinea pig: behavioural dysfunction and hippocampal morphologic change.
Ethanol neuro-behavioural teratogenesis in the guinea pig: behavioural dysfunction and hippocampal morphologic change.
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豚鼠乙醇神经行为致畸：行为功能障碍和海马形态变化。
DOI：
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发表时间：
1993
影响因子：
2.1
通讯作者：
J. Brien
中科院分区：
文献类型：
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作者：
S. Abdollah;M. Catlin;J. Brien
由于豚鼠产前大脑发育广泛，因此在豚鼠中研究了乙醇神经行为致畸作用。我们的目的是在豚鼠后代中研究长期母体给予3和4 gethanol.kg-1 x天-1所产生的行为和海马形态学效应。妊娠豚鼠通过口腔插管接受以下慢性治疗之一：3或4 g乙醇.kg-1 x day-1，两次等分给药，间隔2 h：等热量蔗糖和配对喂养;或水。每种处理获得5窝。在出生后第10、20和60天测定自发活动率，并在出生后第22和62天开始进行自发改变任务的测试。行为测试后，通过对甲酚紫染色冠状切片进行光学显微镜检查，对每个给药组随机选择的豚鼠后代的脑海马进行组织学评估。3和4 g乙醇.kg-1 x day-1方案未限制母体体重增加或后代生长。两种方案均增加了后代的自发活动率，4 g乙醇.kg-1 x day-1方案持续至成年期。两种乙醇方案均不损害自发交替，但4 g乙醇.kg-1 x day-1方案增加了完成试验的百分比。只有4 g乙醇.kg-1 x day-1方案在海马中产生结构损伤，包括CA 1锥体神经元数量减少25%。数据表明，与3 g乙醇.kg-1 x day-1方案相比，4 g乙醇. kg-1 x day-1方案产生更多的行为功能障碍和海马形态学变化。
Ethanol neuro-behavioural teratogenesis was studied in the guinea pig because of its extensive prenatal brain development. Our objective was to study, in the offspring of the guinea pig, behavioural and hippocampal morphologic effects produced by chronic maternal administration of 3 and 4 g ethanol.kg body weight-1 x day-1. Pregnant guinea pig received one of the following chronic treatments via intubation into the oral cavity: 3 or 4 g ethanol.kg-1 x day-1 as two equally divided doses 2 h apart: isocaloric sucrose and pair feeding; or water. Five litters were obtained for each treatment. Locomotor activity rate was determined on postnatal days 10, 20, and 60, and testing of the spontaneous alteration task was conducted beginning on postnatal days 22 and 62. After behavioural testing, the hippocampus of the brain of randomly selected guinea pig offspring of each treatment group was assessed histologically by light microscopic examination of cresyl violet stained coronal sections. The 3 and 4 g ethanol.kg-1 x day-1 regimens did not restrict maternal body weight gain or growth of the offspring. Both regimens increased locomotor activity rate in the offspring, which persisted into adulthood for the 4 g ethanol.kg-1 x day-1 regimen. Neither ethanol regimen impaired spontaneous alternation, but the 4 g ethanol.kg-1 x day-1 regimen increased the percent completed trials. Only the 4 g ethanol.kg-1 x day-1 regimen produced structural injury in the hippocampus, consisting of a 25% decrease in the number of CA1 pyramidal neurons. The data demonstrate that the 4 g ethanol.kg-1 x day-1 regimen produces more behavioural dysfunction and hippocampal morphologic change compared with the 3 g ethanol.kg-1 x day-1 regimen.