Induction of early response genes by hypergravity in cultured mouse osteoblastic cells (MC3T3-E1).
Induction of early response genes by hypergravity in cultured mouse osteoblastic cells (MC3T3-E1).
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在培养的小鼠成骨细胞（MC3T3-E1）中通过超重力诱导早期反应基因。
DOI：
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发表时间：
1994
影响因子：
3.7
通讯作者：
M. Shibanuma
中科院分区：
文献类型：
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作者：
Kiyoshi Nose;M. Shibanuma
低至50g的超重力瞬间刺激培养的小鼠成骨细胞（MC3T3-E1）诱导c-fos和egr-1等早期应答基因，而c-jun的表达则受到轻微影响。c-fos的最大诱导量在90g以上，而egr-1的最大诱导量在50g以下。Staurosporin在0.1 μ m浓度下几乎完全抑制超重力诱导c-fos，但仅部分抑制egr-1的诱导。在用12- o -十四烷醇- 13-乙酸酯预处理的细胞中，超重力诱导的c-fos几乎完全被消除，而egr-1的诱导则不受影响。蛋白激酶C的活性似乎在900g离心的细胞中被激活。这些结果表明，超重刺激了与早期反应基因表达相关的多个信号转导级联反应。
Hypergravity as low as 50g transiently stimulated cultured mouse osteoblastic cells (MC3T3-E1) to induce early response genes such as c-fos and egr-1, whereas expression of c-jun was marginally affected. The maximum induction of c-fos required more than 90g, but egr-1 induction became maximum below 50g. Staurosporin inhibited the induction of c-fos by hypergravity almost completely at a concentration of 0.1 microM, but it inhibited the induction of egr-1 only partially. In cells pretreated with 12-O-tetradecanoylphorbol 13-acetate, induction of c-fos by hypergravity was almost completely abolished, whereas that of egr-1 was not affected. Activity of protein kinase C seemed to be activated in cells centrifuged at 900g. These results indicate that hypergravity stimulates multiple signal transduction cascades that are connected with the expression of early response genes.