Bound polyphenol from foxtail millet bran exhibits an antiproliferative activity in HT-29 cells by reprogramming miR-149-mediated aerobic glycolysis
Bound polyphenol from foxtail millet bran exhibits an antiproliferative activity in HT-29 cells by reprogramming miR-149-mediated aerobic glycolysis
复制标题
来自谷子麸皮的结合多酚通过重编程 miR-149 介导的有氧糖酵解在 HT-29 细胞中表现出抗增殖活性
DOI：
10.1016/j.jff.2019.03.021
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发表时间：
2019-05-01
影响因子：
5.6
通讯作者：
Yang, Dongfeng
中科院分区：
文献类型：
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作者：
Shi, Jiangying;Shan, Shuhua;Yang, Dongfeng
结直肠癌（CRC）是一种难治性肠道肿瘤。癌细胞更喜欢有氧糖酵解来满足快速增殖的要求。因此，发现一种冷抑制有氧糖酵解的生物活性分子，被认为是预防和治疗结直肠癌的有效策略。在本研究中，我们首次发现了一种对HT-29细胞（结肠癌细胞系）及其裸鼠模型具有抗增殖活性的谷子麸皮结合多酚（BPIS）。这些数据进一步揭示了BPIS的抗CRC活性成分和分子机制。在这里，我们发现BPIS可以通过逆转有氧糖酵解在HT-29细胞中发挥抗增殖活性，这种作用是通过BPIS上调miR-149的表达来实现的，BPIS直接靶向c-myc的3'-UTR，从而抑制PKM2介导的有氧糖酵解。因此，BPIS 可以作为一种新的糖酵解抑制剂来增强肠道健康。
Colorectal cancer (CRC) is an intractable intestinal tumor. Cancer cells prefer to aerobic glycolysis to meet the requirements of rapid proliferation. Hence, discovery of a bioactive molecule cold inhibit aerobic glycolysis, which is regarded as a effective strategies for prevention and treatment of CRC. In this study, we first discovered a foxtail millet bran-derived bound polyphenol (BPIS) with anti-proliferative activity in HT-29 cell (colon cancer cell lines) and its nude mice model. The data further indicated the anti-CRC active ingredients and molecular mechanisms of BPIS. Here we revealed that BPIS could act anti-proliferative activity in HT-29 cells depending on reversing aerobic glycolysis, and this effect was achieved by up-regulation of miR-149 expression by BPIS, which directly targeted the 3'-UTR of c-myc, thereby inhibiting PKM2-mediated aerobic glycolysis. Therefore, BPIS might be used as a new inhibitor of glycolysis for enhancing intestines health benefits.