The Schizosaccharomyces pombe actin-related protein, Arp3, is a component of the cortical actin cytoskeleton and interacts with profilin
The Schizosaccharomyces pombe actin-related protein, Arp3, is a component of the cortical actin cytoskeleton and interacts with profilin
复制标题
DOI：
10.1002/j.1460-2075.1996.tb01035.x
复制
发表时间：
1996-12-02
期刊：
影响因子：
11.4
通讯作者：
Gould, KL
中科院分区：
文献类型：
--
作者：
McCollum, D;Feoktistova, A;Gould, KL
编码肌动蛋白相关蛋白 Arp3 的基因首次在裂殖酵母裂殖酵母中被发现，并且是进化上保守的肌动蛋白相关蛋白家族的成员。在这里，我们提出了几个关键发现，定义了 Arp3p 在皮质肌动蛋白细胞骨架功能中的重要作用。首先，arp3 的突变体与 Profilin 和肌动蛋白突变体特异性相互作用，其次，Arp3 定位于极化细胞生长所需的皮质肌动蛋白斑块。第三，arp3基因是细胞周期中肌动蛋白细胞骨架重组所必需的。最后，Arp3蛋白存在于一个大的蛋白复合物中。我们认为，该复合物可能介导粟酒裂殖酵母肌动蛋白斑块上的 profilin 的皮质功能。
The gene encoding the actin-related protein Arp3 was first identified in the fission yeast Schizosaccharomyces pombe and is a member of an evolutionarily conserved family of actin-related proteins. Here we present several key findings that define an essential role for Arp3p in the functioning of the cortical actin cytoskeleton. First, mutants in arp3 interact specifically with profilin and actin mutants, Second, Arp3 localizes to cortical actin patches which are required for polarized cell growth. Third, the arp3 gene is required for the reorganization of the actin cytoskeleton during the cell cycle, Finally, the Arp3 protein is present in a large protein complex. We believe that this complex may mediate the cortical functions of profilin at actin patches in S. pombe.