Photodynamic treatment has chondroprotective effects on articular cartilage.
Photodynamic treatment has chondroprotective effects on articular cartilage.
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光动力治疗对关节软骨具有软骨保护作用。
DOI：
10.1016/s0736-0266(01)00113-9
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Towle,ChristineA
中科院分区：
文献类型：
--
作者：
Sullivan,LawrenceG;Hasan,Tayyaba;Wright,Marianne;Mankin,HenryJ;Towle,ChristineA
骨关节炎（OA）是一种致残性关节疾病，目前尚无治愈方法。其特征在于关节软骨的破坏。正在探索的一种保护OA软骨的策略是给予转化生长因子-β，其在体外拮抗由分解代谢刺激剂如白细胞介素-1（IL-1）引发的软骨降解。与选择性递送生长因子至软骨细胞相关的问题、对关节组织的不良副作用以及短的生物半衰期促使我们探索旨在激活作为潜在复合物储存在软骨中的转化生长因子-β的模式。光动力疗法是一种两步的组织敏化方案，使用称为光敏剂的光活化化学物质，然后以一定间隔用适当波长的可见光照射。生物效应通常通过氧依赖性光化学引起，而不产生热量。转化生长因子-β1可以通过氧化机制激活，促使我们探索光动力技术是否可以用于调节软骨代谢。通过与氯6-琥珀酰化多聚赖氨酸缀合物孵育并用1-2 J/cm 2红光（λmax= 671 nm）照射来光敏化牛关节软骨的软骨。这种两步方案显著抑制了IL-1刺激的蛋白聚糖降解，同时增加了培养基中的潜伏和活性转化生长因子-β1。这项研究可能会导致微创光动力疗法的发展，其中光被传递到局部激活光敏软骨中的软骨保护程序在OA的背景下。© 2002骨科研究学会。由爱思唯尔科技有限公司出版。保留所有权利。
Osteoarthritis (OA) is a disabling joint disease for which there is currently no cure. It is characterized by the destruction of articular cartilage. One strategy that is being explored for protecting the cartilage in OA is the administration of transforming growth factor‐β, which in vitro antagonizes cartilage degradation initiated by catabolic stimulants such as interleukin‐1 (IL‐1). The problems associated with selective delivery of the growth factor to chondrocytes, undesirable side‐effects on joint tissues, and short biological half‐life have led us to explore modalities aimed at activating transforming growth factor‐β that is stored in the cartilage as latent complexes. Photodynamic therapy is a two‐step protocol of tissue sensitization with a light‐activatable chemical called a photosensitizer followed at some interval by irradiation with the appropriate wavelength visible light. Biological effects are typically elicited through oxygen‐dependent photochemistry without heat generation. Transforming growth factor‐β1 can be activated by oxidative mechanism(s), prompting us to explore whether photodynamic technology can be harnessed to modulate cartilage metabolism. Disks of bovine articular cartilage were photosensitized by incubation with a chlorine6‐succinylated polylysine conjugate and irradiated with 1–2 J/cm2red light (λmax= 671 nm). This two‐step regimen dramatically inhibited IL‐1‐stimulated proteoglycan degradation and concomitantly increased latent and active transforming growth factor‐β1 in culture medium. This research may lead to the development of minimally invasive photodynamic therapy in which light is delivered to locally activate a chondroprotective program in photosensitized cartilage in the context of OA. © 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.
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