A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode
A Highly Sensitive Amperometric Glutamate Oxidase Microbiosensor Based on a Reduced Graphene Oxide/Prussian Blue Nanocube/Gold Nanoparticle Composite Film-Modified Pt Electrode
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DOI：
10.3390/s20102924
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发表时间：
2020-05-01
期刊：
影响因子：
3.9
通讯作者：
Zhang, Shaomin
中科院分区：
文献类型：
--
作者：
Chen, Jing;Yu, Qiwen;Zhang, Shaomin
一种简单的方法，仅依赖于电化学工作站已被调查，以制作一个高灵敏度的谷氨酸微生物传感器的潜在神经科学应用。在这项研究中，为了开发高灵敏度的谷氨酸电极，100 μ m的铂丝被改性的电化学沉积的金纳米粒子，普鲁士蓝纳米立方体，并减少氧化石墨烯片，这增加了电活性表面积;和壳聚糖层，这提供了一个合适的环境来结合谷氨酸氧化酶。描述了制备过程和分析条件的优化。采用场发射扫描电子显微镜、交流阻抗谱和循环伏安法对修饰电极进行了表征。结果显示其对谷氨酸检测的灵敏度（LOD = 41.33 nM）、足够的线性（50 nM-40 μ M）、优越的重现性（RSD = 4.44%）和延长的稳定性（超过30次重复电位扫描，两周的寿命）。由于谷氨酸在神经传递和脑功能中的重要作用，这种小尺寸、高灵敏度的谷氨酸电极在神经科学研究中是一种很有前途的工具。
A simple method that relies only on an electrochemical workstation has been investigated to fabricate a highly sensitive glutamate microbiosensor for potential neuroscience applications. In this study, in order to develop the highly sensitive glutamate electrode, a 100 mu m platinum wire was modified by the electrochemical deposition of gold nanoparticles, Prussian blue nanocubes, and reduced graphene oxide sheets, which increased the electroactive surface area; and the chitosan layer, which provided a suitable environment to bond the glutamate oxidase. The optimization of the fabrication procedure and analytical conditions is described. The modified electrode was characterized using field emission scanning electron microscopy, impedance spectroscopy, and cyclic voltammetry. The results exhibited its excellent sensitivity for glutamate detection (LOD = 41.33 nM), adequate linearity (50 nM-40 mu M), ascendant reproducibility (RSD = 4.44%), and prolonged stability (more than 30 repetitive potential sweeps, two-week lifespan). Because of the important role of glutamate in neurotransmission and brain function, this small-dimension, high-sensitivity glutamate electrode is a promising tool in neuroscience research.