Enzyme-linked immunosorbent assay for detection of T-2 toxin.
Enzyme-linked immunosorbent assay for detection of T-2 toxin.
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用于检测 T-2 毒素的酶联免疫吸附测定。
DOI：
10.1515/bchm2.1982.363.2.1437
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
K. Dose
中科院分区：
文献类型：
--
作者：
H. Peters;M. Dierich;K. Dose
建立了检测T-2毒素的微量试板酶免疫分析方法。将Haptenic T-2琥珀酸半酯固定在涂有聚D-赖氨酸的聚苯乙烯表面。加入兔抗T-2毒素血清。通过加入抗兔免疫球蛋白/过氧化物酶结合物来检测第一抗体的结合。底物2，2‘-azinobis(3-ethyl-2，3-dihydrobenzothiazole-6-sulfonate)/H2O2，转换后，在414nm处监测吸光度。在第一个孵育步骤中，游离T-2毒素的存在导致吸光度随浓度的降低而降低。最低检出限为2pg/次。用ELISA法测定T-2毒素抗血清与其他三环烯类化合物的交叉反应比用放射免疫分析技术测定的结果弱。
A microtest plate enzyme immunoassay has been developed for the detection of T-2 toxin. Haptenic T-2 hemisuccinate was immobilized on polystyrene surfaces coated with poly(D-lysine). rabbit antisera against T-2 toxin were added. The binding of the first antibody was detected by addition of an anti-rabbit-immunoglobulin/peroxidase conjugate. After conversion of the substrate 2,2'-azinobis(3-ethyl-2,3-dihydrobenzothiazole-6-sulfonate)/H2O2, the absorbance at 414 nm was monitored. Presence of free T-2 toxin during the first incubation step led to a concentration-dependent lowering of absorbance. The lower detection limit was at about 2 pg per assay. The cross reactivity of T-2 toxin antiserum with other trichothecenes, as determined by ELISA, is weaker than that reported by other authors using a radio-immunoassay technique.