Sample displacement chromatography as a method for purification of proteins and peptides from complex mixtures.
Sample displacement chromatography as a method for purification of proteins and peptides from complex mixtures.
复制标题
样品置换色谱作为从复杂混合物中纯化蛋白质和肽的方法。
DOI：
10.1016/j.chroma.2012.03.046
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Josic,Djuro
中科院分区：
文献类型：
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作者：
SrajerGajdosik,Martina;Clifton,James;Josic,Djuro
反相和离子交换模式的样品置换色谱（SDC）大约在20年前被引入。该方法利用了样品混合物中组分的相对结合亲和力。在加载过程中，不同样品组分之间存在着固定相表面吸附的竞争。SDC首先用于蛋白质的制备纯化。后来，证明了这种色谱也可以在离子交换、亲和和疏水相互作用模式下进行。还表明，在分析和制备规模上，SDC可以在单体和膜基支架上进行。最近，离子交换和疏水相互作用模式下的SDC也被成功地用于去除单克隆抗体制剂中的微量蛋白质和从人血浆中富集低丰度蛋白质。本文介绍了SDC的基本原理，并讨论了SDC在微量分析、分析和制备等领域分离蛋白质和多肽的潜力。
Sample displacement chromatography (SDC) in reversed-phase and ion-exchange modes was introduced approximately twenty years ago. This method takes advantage of relative binding affinities of components in a sample mixture. During loading, there is a competition among different sample components for the sorption on the surface of the stationary phase. SDC was first used for the preparative purification of proteins. Later, it was demonstrated that this kind of chromatography can also be performed in ion-exchange, affinity and hydrophobic-interaction mode. It has also been shown that SDC can be performed on monoliths and membrane-based supports in both analytical and preparative scale. Recently, SDC in ion-exchange and hydrophobic interaction mode was also employed successfully for the removal of trace proteins from monoclonal antibody preparations and for the enrichment of low abundance proteins from human plasma. In this review, the principals of SDC are introduced, and the potential for separation of proteins and peptides in micro-analytical, analytical and preparative scale is discussed.