Serotonergic modulation of respiratory motor output during tadpole development
Serotonergic modulation of respiratory motor output during tadpole development
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DOI：
10.1152/japplphysiol.00104.2002
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发表时间：
2002-09-01
影响因子：
3.3
通讯作者：
Gulemetova, R
中科院分区：
文献类型：
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作者：
Kinkead, R;Belzile, O;Gulemetova, R
为了检验这一假设，5-羟色胺（5-羟色胺; 5-HT）受体激活eleveling呼吸运动输出的年龄依赖性的变化，我们比较了5-HT浴应用（5-HT浓度= 0.5-25 μ M）的影响，在体外脑干制备前和变性后牛蛙蝌蚪。记录与鳃和肺通气相关的运动输出表明，5-HT在两组中都能剂量依赖性地抑制鳃爆发频率。与此相反，肺爆发频率反应是阶段依赖性的，在低5-HT浓度（小于或等于0.5 μ M）的肺爆发频率增加，观察到只有在postmetamorphic组。较高的5-HT浓度降低肺爆发频率在所有的准备工作。鳃爆发频率衰减介导的（至少部分）5-HT 1A受体激活的年龄依赖性的方式。我们的结论是，呼吸运动输出的α-肾上腺素能调制1）在蝌蚪发育过程中发生变化，2）鳃和肺通气是不同的。
To test the hypothesis that serotonin (5-hydroxytryptamine; 5-HT)-receptor activation elicits age-dependent changes in respiratory motor output, we compared the effects of 5-HT bath application (5-HT concentration = 0.5-25 muM) onto in vitro brain stem preparations from pre- and postmetamorphic bullfrog tadpoles. Recording of motor output related to gill and lung ventilation showed that 5-HT elicits a dose-dependent depression of gill burst frequency in both groups. In contrast, the lung burst frequency response was stage dependent; an increase in lung burst frequency at low 5-HT concentration (less than or equal to0.5 muM) was observed only in the postmetamorphic group. Higher 5-HT concentrations decreased lung burst frequency in all preparations. Gill burst frequency attenuation is mediated (at least in part) by 5-HT1A-receptor activation in an age-dependent fashion. We conclude that serotonergic modulation of respiratory motor output 1) changes during tadpole development and 2) is distinct for gill and lung ventilation.