Apparent membrane pore-formation by Portuguese Man-of-war (Physalia physalis) venom in intact cultured cells
Apparent membrane pore-formation by Portuguese Man-of-war (Physalia physalis) venom in intact cultured cells
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DOI：
10.1016/s0041-0101(02)00138-1
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发表时间：
2002-09-01
期刊：
影响因子：
2.8
通讯作者：
Hessinger, DA
中科院分区：
文献类型：
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作者：
Edwards, LP;Whitter, E;Hessinger, DA
Fura-2的细胞内比率显微荧光分析表明，低剂量的葡萄牙僧帽水母（酸浆）毒液导致培养的L-929细胞内钙积累的线性增加。钙的流入之前是一个滞后期，这是相对独立的毒液浓度，除了在非常低的浓度。用毒液处理的L-929和GH（4）C（1）细胞的负染膜制备物的电子显微镜照片显示直径为10-80 nm的损伤。这些病变的数量和直径与毒液浓度相关。该毒液在GH（4）C（1）细胞中形成损伤的浓度比在L-929细胞中低得多。渗透保护剂如蔗糖和聚乙二醇（PEG）降低了从毒液处理的细胞释放乳酸脱氢酶（LDH）的程度，其中分子量较高的PEG比蔗糖对LDH释放的抑制更大。这些结果意味着，Man-of-war毒液在靶细胞的膜中产生孔样结构，这导致胶体渗透溶胀，随后释放细胞内蛋白和细胞溶解。(C)2002爱思唯尔科技有限公司版权所有。
Intracellular, ratiometric microfluorimetry with fura-2 reveals that low doses of Portuguese Man-of-war (Physalia physalis) venom cause a linear increase in intracellular calcium accumulation by cultured L-929 cells. The influx of calcium is preceded by a lag period that is relatively independent of venom concentration, except at very low concentrations. Electron micrographs of negatively stained preparations of membranes from venom-treated L-929 and GH(4)C(1) cells exhibit 10-80 nm diameter lesions. The number and diameter of these lesions correlate with venom concentration. The venom forms lesions in GH(4)C(1) cells at much lower concentrations than in L-929 cells. Osmotic protectants such as sucrose and polyethylene glycol (PEG), reduce the extent of lactate dehydrogenase (LDH) release from venom-treated cells with the higher molecular weight PEG causing a greater inhibition of LDH release than sucrose. These results imply that Man-of-war venom produces pore-like structures in the membranes of target cells, which leads to colloid osmotic swelling with subsequent release of intracellular proteins and cell lysis. (C) 2002 Elsevier Science Ltd. All rights reserved.