Microbiological implications of electric field effects VII. Stimulation of plasmid transformation of Bacillus cereus protoplasts by electric field pulses
Microbiological implications of electric field effects VII. Stimulation of plasmid transformation of Bacillus cereus protoplasts by electric field pulses
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电场效应的微生物学意义 VII．
DOI：
10.1002/jobm.19830230915
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发表时间：
1983
影响因子：
3.1
通讯作者：
R. Grigorova
中科院分区：
文献类型：
--
作者：
N. Shivarova;W. Förster;H. Jacob;R. Grigorova
本研究的目的是检验电场脉冲对蜡状芽孢杆菌完整细胞和原生质体的存活和对这些原生质体与苏云金芽孢杆菌转化体质粒DNA的转化频率的作用。- B。蜡状细胞和原生质体对微秒范围内的高电场脉冲非常耐受。B.蜡状原生质体与来自B的质粒。用电场脉冲技术可使苏云金杆菌（pUB 110）的杀虫活性提高约一个数量级。转化体是稳定的，pUB 110作为染色体外元件被保留。
The aim of this study was to check the action of electric field pulses (1) on the survival of intact cells and protoplasts of Bacillus cereus and (2) on the transformation frequency of these protoplasts with plasmid DNA from Bacillus thuringiensis transformants. – B. cereus cells and protoplasts are very resistant to high electric field pulses in the microsecond range. The transformation frequency of B. cereus protoplasts with plasmids from B. thuringiensis (pUB 110) can be increased by about one order of magnitude with the electric field pulse technique. The transformants are stable and pUB 110 is preserved as an extrachromosomal element.