Determination of the promoter strength of the gene encoding Escherichia coli heat-stable enterotoxin II.
Determination of the promoter strength of the gene encoding Escherichia coli heat-stable enterotoxin II.
复制标题
编码大肠杆菌热稳定肠毒素II的基因启动子强度的测定。
DOI：
10.1128/jb.169.4.1740-1744.1987
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发表时间：
1987
影响因子：
3.2
通讯作者：
Lee,CH
中科院分区：
文献类型：
--
作者：
Spandau,DF;Lee,CH
We studied the promoter strength of the gene encoding the Escherichia coli heat-stable enterotoxin II (STII). The promoter region and a portion of the 5' coding sequence of the STII gene were fused to the lacZ gene so that the production of beta-galactosidase was under the control of the STII gene promoter. The strength of the STII gene promoter was compared with that of the ompF and lac operons, which were similarly fused to the lacZ gene. The beta-galactosidase produced by the hybrid genes was assayed in vitro by using cell extracts. The mRNA transcribed by each promoter was assayed by Northern blot analysis and by in vitro transcription. The results suggest that the STII gene is regulated by a relatively weak promoter.
