Porous copper- and lithium-doped nano-hydroxyapatite composite scaffold promotes angiogenesis and bone regeneration in the repair of glucocorticoids-induced osteonecrosis of the femoral head
Porous copper- and lithium-doped nano-hydroxyapatite composite scaffold promotes angiogenesis and bone regeneration in the repair of glucocorticoids-induced osteonecrosis of the femoral head
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DOI：
10.1088/1748-605x/ac246e
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发表时间：
2021-11-01
影响因子：
4
通讯作者：
Kang, Pengde
中科院分区：
文献类型：
--
作者：
Li, Bohua;Lei, Yan;Kang, Pengde
糖皮质激素性股骨头坏死是一种常见的难治性疾病。本研究旨在合成纳米羟基磷灰石-铜-锂（Cu-Li-nHA）复合多孔支架，通过调控Wnt beta-catenin和HIF-1 alpha/VEGF通路，促进成骨和血管生成功能，修复GIONFH。通过一系列的体内外实验，对支架材料的理化性质进行了表征，并对其成骨和血管生成作用进行了测试。结果表明，0.25%的Cu-Li-nHA支架具有最高的体外力学性能和生物相容性。0.25%的Cu-Li-nHA支架在体内可显著促进GIONFH兔骨缺损处的新骨形成。此外，该支架可以增加成骨和血管生成因子的表达，沿着Wnt β-catenin和HIF-1 alpha/VEGF途径中的因子在体外和体内的激活。结论：0. 25% Cu-Li-nHA支架可通过上调Wnt β-catenin和HIF-1 alpha/VEGF通路促进GIONFH的成骨和血管生成，有利于GIONFH的修复。
Glucocorticoids-induced osteonecrosis of the femoral head (GIONFH) is a common refractory disease. In the present study, we aimed to synthesize the nano-hydroxyapatite-copper-lithium (Cu-Li-nHA) composite porous scaffold to promote osteogenesis and angiogenesis functions to repair GIONFH by regulating the Wnt beta-catenin and HIF-1 alpha/VEGF pathways. The physicochemical property of the scaffold was characterized and their osteogenic and angiogenic effects were tested through a serial of experiments in vitro and in vivo. Results showed that 0.25% Cu-Li-nHA scaffolds possessed the highest mechanical and biocompatibility in vitro. Then the 0.25% Cu-Li-nHA scaffolds significantly enhanced the new bone formation on defects in GIONFH rabbits in vivo. Moreover, the scaffold could increase the expression of osteogenic and angiogenic factors along with the activation of factors in Wnt beta-catenin and HIF-1 alpha/VEGF pathways in vitro and in vivo. In conclusion, the 0.25% Cu-Li-nHA scaffold could improve the osteogenesis and angiogenesis by upregulating the Wnt beta-catenin and HIF-1 alpha/VEGF pathways which benefited to repair the GIONFH in rabbit models.