Effects of Ascorbic, Acid on Gingival Melanin Pigmentation In Vitro and In Vivo
Effects of Ascorbic, Acid on Gingival Melanin Pigmentation In Vitro and In Vivo
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DOI：
10.1902/jop.2009.080409
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发表时间：
2009-02-01
影响因子：
4.3
通讯作者：
Yoshie, Hiromasa
中科院分区：
文献类型：
--
作者：
Shimada, Yasuko;Tai, Hideaki;Yoshie, Hiromasa
背景：即使没有严重的医学问题，牙龈黑色素沉着也可能引起美学问题。作为黑色素形成的抑制剂，抗坏血酸通常用于治疗皮肤黑色素沉着。因此，本研究探讨了抗坏血酸对牙龈黑色素沉着的影响，在体外和在vivo.Methods：抗坏血酸对黑色素形成的影响进行了评估，在体外B16小鼠黑色素瘤细胞和三维人体皮肤模型。此外，进行临床试验以研究含有抗坏血酸2-葡萄糖苷的凝胶（AS-G凝胶）对牙龈黑色素沉着的抑制作用。本研究采用双盲、分口设计，对73名对称性牙龈黑色素沉着的受试者进行了研究。AS-G凝胶应用于一侧牙龈12周，而安慰剂凝胶应用于另一侧作为对照。每4周用分光光度法测定牙龈亮度（L*）值，以获得黑色素沉着的客观量度。结果：抗坏血酸显著抑制B16小鼠黑色素瘤细胞酪氨酸酶活性和黑色素形成（P
Background: Gingival melanin pigmentation may cause esthetic concerns, even if no serious medical problem is present. As an inhibitor of melanin formation, ascorbic acid is often used to treat skin melanin pigmentation. Thus, the present study investigated the effects of ascorbic acid on gingival melanin pigmentation in vitro and in vivo.Methods: The effects of ascorbic acid on melanin formation were evaluated in vitro in B16 mouse melanoma cells and three-dimensional human skin models. In addition, a clinical trial was performed to investigate the inhibitory effects of a gel containing ascorbic acid 2-glucoside (AS-G gel) on gingival melanin pigmentation. This study used a double-masked, split-mouth design on 73 subjects with symmetric gingival melanin pigmentation. AS-G gel was applied to one side of the gingiva for 12 weeks, whereas placebo gel was applied to the other side as a control. Luminance (L*)-value, which describes the lightness of gingiva, was determined by spectrophotometry to obtain an objective measure of melanin pigmentation every 4 weeks.Results: Ascorbic acid significantly inhibited tyrosinase activity and melanin formation in B16 mouse melanoma cells (P