Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues
Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues
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DOI：
10.1117/1.jbo.24.4.046501
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发表时间：
2019-04-01
影响因子：
3.5
通讯作者：
Solgaard, Olav
中科院分区：
文献类型：
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作者：
Landry, Joseph R.;Itoh, Ryosuke;Solgaard, Olav
我们展示了使用可调谐结构照明（SI）频率来优化散射样本的图像质量的光片荧光显微镜中改进的光学切片。 SI 图案是使用一维空间光调制器相干生成的，以实现最大图案对比度，并且图案空间频率可调节至我们检测目标的非相干截止频率的一半。在这个频率下，我们证明背景降低了 2 个数量级。 (C) 作者。由 SPIE 根据 Creative Commons Attribution 4.0 Unported 许可证发布。
We demonstrate improved optical sectioning in light sheet fluorescence microscopy using tunable structured illumination (SI) frequencies to optimize image quality in scattering specimens. The SI patterns are generated coherently using a one-dimensional spatial light modulator for maximum pattern contrast, and the pattern spatial frequency is adjustable up to half the incoherent cutoff frequency of our detection objective. At this frequency, we demonstrate background reductions of 2 orders of magnitude. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.