Bifidobacterium longum, a lactic acid-producing intestinal bacterium inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis
Bifidobacterium longum, a lactic acid-producing intestinal bacterium inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis
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DOI：
10.1093/carcin/18.4.833
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发表时间：
1997-04-01
期刊：
影响因子：
4.7
通讯作者：
Reddy, BS
中科院分区：
文献类型：
--
作者：
Singh, J;Rivenson, A;Reddy, BS
人体结肠是一个复杂的微生物生态系统，包括数百种细菌，其中一些肠道细菌对宿主有益，并已显示出抗突变和抗癌特性。我们研究了长双歧杆菌（Bifidobacterium longum），一种产乳酸的肠杆菌，并分析了乳酸培养物对结肠癌发生过程中结肠粘膜和/或肿瘤细胞增殖、ODC活性和ras-p21癌蛋白表达的调节作用。雄性F344大鼠喂食含有0或2%长B.longum和s.c.以15 mg/kg体重的剂量每周一次持续2周施用溶解在生理盐水中的氧化偶氮甲烷（AOM），载体对照接受等体积的生理盐水s.c.动物维持对照或实验饮食直至研究终止。用于分析细胞增殖的动物在第二次AOM注射后20周处死，而用于结肠肿瘤分析和ODC活性和ras-p21表达测量的动物在最后一次AOM注射后40周处死，数据表明，饮食给予长B.longum的冻干培养物导致结肠肿瘤发病率和肿瘤多样性的显著抑制，并且还降低了结肠肿瘤的发生率和肿瘤多样性。结果还显示，摄取长B.longum显著抑制AOM诱导的细胞增殖、ODC活性和ras-p21癌蛋白的表达。数据表明，口服益生菌B.longum发挥强的抗肿瘤活性，如通过调节结肠癌的中间生物标志物所指示的，并因此降低肿瘤结果。
The human colon can be described as a complex microbial ecosystem, comprising several hundred bacterial species, Some of these enteric bacteria are beneficial to the host and have been shown to exert antimutagenic and anticarcinogenic properties, We have investigated the colon tumor inhibitory activity of Bifidobacterium longum, a lactic acid-producing enterobacterium, The modifying effects of this lactic culture on colonic mucosal and/or tumor cell proliferation, ODC activity and ras-p21 oncoprotein expression in colon carcinogenesis were also analyzed. Male F344 rats were fed a modified AIN-76A diet containing 0 or 2% lyophilized cultures of B.longum and s.c. administered azoxymethane (AOM) dissolved in normal saline at a dose of 15 mg/kg body wt, once weekly for 2 weeks, Vehicle controls received an equal volume of normal saline s.c. Animals were maintained on control or experimental diets until termination of the study. Animals intended for analysis of cell proliferation were killed 20 weeks after the second AOM injection, whereas animals intended for colon tumor analysis and measurement of ODC activity and ras-p21 expression were killed 40 weeks after the last AOM injection, The data demonstrate that dietary administration of lyophilized cultures of B.longum resulted in significant suppression of colon tumor incidence and tumor multiplicity and also reduced tumor volume, Results also revealed that ingestion of B.longum significantly inhibited AOM-induced cell proliferation, ODC activity and expression of ras-p21 oncoprotein, Data suggest that oral administration of probiotic B.longum exerts strong antitumor activity, as indicated by modulation of the intermediate biomarkers of colon cancer, and consequently reduced tumor outcome.