Integrated analysis of expression and genome alteration reveals putative amplified target genes in esophageal cancer.
Integrated analysis of expression and genome alteration reveals putative amplified target genes in esophageal cancer.
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DOI：
10.3892/or.18.2.465
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发表时间：
2007-08
期刊：
影响因子：
4.2
通讯作者：
Takashi Sugimoto;M. Arai;H. Shimada;A. Hata;N. Seki
中科院分区：
文献类型：
--
作者：
Takashi Sugimoto;M. Arai;H. Shimada;A. Hata;N. Seki
微阵列和比较基因组杂交（CGH）研究提供了广泛的信息，食管癌，但基因表达和拷贝数改变之间的相关性在很大程度上是未知的。为筛选食管癌扩增靶基因，采用经典的计算全息（CGH）和寡核苷酸芯片技术，对10个食管鳞癌细胞系的DNA拷贝数和基因表达进行了平行检测。随后使用信噪比分析整合基因表达和拷贝数数据。结果显示，一组97个基因的表达水平升高，这是由于拷贝数增加。这组基因包括先前报道的在癌症中过表达的基因以及几个与拷贝数升高相关的新基因。这些基因参与基本的细胞过程（例如，转录调节、信号转导、细胞增殖、细胞周期和细胞分化），其也可对癌症发展产生影响。因此，DNA和RNA谱的整合为发现参与食管癌发展和进展的基因提供了一个高产的切入点。
Microarray and comparative genomic hybridization (CGH) studies have provided a wide range of information about esophageal cancer, but the correlations between gene expression and copy number alteration are largely unknown. To identify putative amplification target genes in esophageal cancer, a survey of parallel DNA copy number and gene expression in 10 esophageal squamous cell carcinoma (ESCC) cell lines was performed using classical CGH and oligonucleotide microarrays. The gene expression and copy number data were subsequently integrated using signal-to-noise ratio analysis. The results revealed a set of 97 genes with elevated expression levels that were attributable to increased copy number. The set included genes previously reported as overexpressed in cancer as well as several novel genes associated with copy number elevation. These genes are involved in essential cellular processes (e.g., regulation of transcription, signal transduction, cell proliferation, the cell cycle and cell differentiation) that can also have an impact on cancer development. Thus, the integration of DNA and RNA profiles provides a highly productive entry point for the discovery of genes involved in the development and progression of esophageal cancer.