THE SIGNIFICANCE OF SODIUM CHLORIDE IN STUDIES OF STAPHYLOCOCCI
THE SIGNIFICANCE OF SODIUM CHLORIDE IN STUDIES OF STAPHYLOCOCCI
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DOI：
10.1128/jb.50.2.201-203.1945
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发表时间：
1945-01-01
影响因子：
3.2
通讯作者：
CHAPMAN, GH
中科院分区：
文献类型：
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作者：
CHAPMAN, GH
将75克NaCl加入1升Bacto酚红甘露醇琼脂中，为葡萄球菌的分离提供了极好的培养基。致病型在36小时内产生大菌落。被黄色区域包围;非致病性类型产生没有黄色区域的小菌落。其他细菌很少在培养基上生长。每升含75克NaCl的蛋白糖乳糖琼脂。是很好的股票文化。它增强了显色和凝固酶的生产，并减少了“简并”解离的比例。血浆葡萄球菌的回收率远优于以往任何方法。
75 grams NaCl added to 1 liter of Bacto phenol red mannitol agar provided an excellent medium for the isolation of staphylococci. Pathogenic types produced large colonies in 36 hrs. surrounded by yellow zones; nonpathogenic types produced small colonies without yellow zones. Other bacteria rarely grew on the medium. Proteose lactose agar containing 75 grams NaCl per 1. was excellent for stock cultures. It enhanced chromogenesis and coagulase production and reduced the proportion of "degenerate" dissociants. The recovery of plasma coagulating staphylococci was far better than by any previous method.