Salmonellosis: Serotypes, prevalence and multi-drug resistant profiles of Salmonella enterica in selected poultry farms, Kwara State, North Central Nigeria
Salmonellosis: Serotypes, prevalence and multi-drug resistant profiles of Salmonella enterica in selected poultry farms, Kwara State, North Central Nigeria
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DOI：
10.4102/ojvr.v86i1.1667
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发表时间：
2019-01-01
影响因子：
2.7
通讯作者：
Akorede, Ganiu J.
中科院分区：
文献类型：
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作者：
Ahmed, Akeem O.;Raji, Moshood A.;Akorede, Ganiu J.
沙门氏菌病是全球家禽业面临的主要威胁。本研究的目的是调查沙门氏菌污染的水平，并确定从夸拉州（尼日利亚南部和北方地区之间的过渡州）选定的家禽养殖场获得的分离株的耐药性模式。2017年1月至8月期间，从家禽环境中收集了900份样本，显然包括健康和死亡的鸟类。使用标准细菌学方法分离和鉴定沙门氏菌。所有假定沙门氏菌分离株进行血清分型，并使用11种不同的抗菌药物进行抗菌药物敏感性测试。共获得58株沙门氏菌分离株（6.4%），仅在活禽中分离率有统计学意义（p < 0.05）。分离株由13个血清型组成。从所有三种样本类型中分离出三种主要血清型，即肠道沙门氏菌血清型6.7：d：-（29.0%）、阿加马沙门氏菌（28.0%）和鼠伤寒沙门氏菌（16.0%）。本研究还分离出罕见血清型，如沙门氏菌奥尔巴尼、沙门氏菌科林代尔、沙门氏菌伊斯坦布尔、沙门氏菌拉罗谢尔、沙门氏菌尼日利亚和沙门氏菌猎户座。一般观察到高频率的耐药，所有分离株对氨苄西林、头孢噻肟和头孢他啶的耐药率均为100%。本研究记录了第一个优势分离的S。enterica ser.6.7：d：-和S.鸡的阿加马。它还记录了高频率的氟喹诺酮和头孢菌素耐药的菌株，表明在环境中存在的选择性压力。应启动针对沙门氏菌的控制和有针对性的干预措施以及鸡中频繁发生的抗菌素耐药性，以防止这种微生物的传播。
Salmonellosis is a major threat facing the poultry industry globally. This study was conducted to investigate the level of Salmonella contaminations and determine the resistance pattern of isolates obtained from selected poultry farms in Kwara State, a transition state between southern and northern regions of Nigeria. A total of 900 samples were collected between January and August 2017, from the poultry environment, apparently including healthy and dead birds. Salmonella was isolated and identified using standard bacteriological methods. All presumptive Salmonella isolates were serotyped and tested for antimicrobial susceptibility using 11 different antimicrobials. A total of 58 (6.4%) Salmonella isolates were obtained, and the isolation rate was only statistically significant (p < 0.05) in live birds. The isolates comprised of 13 serovars. The three predominant serovars, Salmonella enterica ser. 6.7: d:- (29.0%), Salmonella Agama (28.0%) and Salmonella Typhimurium (16.0%), were isolated from all three sample types. Rare serovars like Salmonella Albany, Salmonella Colindale, Salmonella Istanbul, Salmonella Larochelle, Salmonella Nigeria and Salmonella Orion were also isolated in this study. A high frequency of resistance was generally observed with all the isolates exhibiting a total of (100%) resistance to ampicillin, cefotaxime and ceftazidime. This study documents the first predominant isolation of S. enterica ser. 6.7: d:- and S. Agama from chickens. It also documents the high frequency of fluoroquinolone and cephalosporins resistance of the isolates indicating the presence of selective pressure in the environment. Controls and targeted interventions against Salmonella and the frequent occurrence of antimicrobial resistance in chickens should be initiated to prevent the spread of this organism.