Molecular cloning and sexually dimorphic expression of DM-domain genes in Daphnia magna
Molecular cloning and sexually dimorphic expression of DM-domain genes in Daphnia magna
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DOI：
10.1016/j.ygeno.2007.09.002
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发表时间：
2008-01-01
期刊：
影响因子：
4.4
通讯作者：
Iguchi, Taisen
中科院分区：
文献类型：
--
作者：
Kato, Yasuhiko;Kobayashi, Kaoru;Iguchi, Taisen
众所周知，大水蚤会根据环境在有性繁殖和无性繁殖之间切换。当处于食物、光照和人口密度的最佳环境时，它进行无性繁殖。一旦环境恶化，它就会将繁殖策略从无性繁殖转变为有性繁殖。然而，环境性别决定的分子基础在很大程度上是未知的。为了了解水蚤环境性别决定的分子机制，分离与环境性别决定相关的基因是十分必要的。由于dm结构域基因是众所周知的性别相关基因，我们旨在从水蚤中鉴定dm结构域基因。利用水蚤cDNA对DM保守结构域进行简并PCR，鉴定出黑腹果蝇DMRT11E、DMRT93B和DMRT99B对应的3个DM结构域基因，在生殖腺中定量表达分析发现DMRT93B仅在睾丸中表达。这一发现有助于更好地理解从无性到有性生命周期的转换机制，这取决于环境。(C) 2007爱思唯尔公司版权所有。
Daphnia magna is known to switch between sexual and asexual reproduction depending on the environment. It reproduces asexually when in an optimal environment for food, photoperiod, and population density. Once the environment declines, it changes reproductive strategy from asexual to sexual reproduction. However, the molecular bases of environmental sex determination are largely unknown. To understand the molecular mechanisms of environmental sex determination in Daphnia, it is essential to isolate the genes related to sex determination. As DM-domain genes are well known as sex-related genes, we aimed to identify DM-domain genes from Daphnia. Based on degenerate PCR of conserved DM domains using Daphnia cDNA, we identified three DM-domain genes that corresponded to DMRT11E, DMRT93B, and DMRT99B of Drosophila melanogaster Quantitative gene expression analysis in gonads revealed that DMRT93B was expressed only in the testis. This finding contributes to an improved understanding of the switching mechanism from an asexual to a sexual life cycle depending on the environment. (C) 2007 Elsevier Inc. All rights reserved.