Detection of swelling of single isolated mitochondrion with optical microscopy
Detection of swelling of single isolated mitochondrion with optical microscopy
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DOI：
10.1364/boe.5.000848
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发表时间：
2014-03-01
影响因子：
3.4
通讯作者：
Ohta, Yoshihiro
中科院分区：
文献类型：
--
作者：
Morikawa, Daisuke;Kanematsu, Keita;Ohta, Yoshihiro
渗透负荷下的体积调节是细胞和细胞器最基本的功能之一。然而，尚未开发出检测单个细胞器体积变化的有效方法。在这里，我们提出了一种基于光穿过线粒体的透射率来检测水溶液中单个孤立线粒体体积变化的新技术。我们发现，添加低渗溶液和 Ca2+ 后，分别有 70% 和 21% 的线粒体肿胀。这些结果显示了本技术检测单个小细胞器（例如线粒体）的生理体积变化的潜力。 (C) 2014年美国光学学会
Volume regulation under osmotic loading is one of the most fundamental functions in cells and organelles. However, the effective method to detect volume changes of a single organelle has not been developed. Here, we present a novel technique for detecting volume changes of a single isolated mitochondrion in aqueous solution based on the transmittance of the light through the mitochondrion. We found that 70% and 21% of mitochondria swelled upon addition of a hypotonic solution and Ca2+, respectively. These results show the potential of the present technique to detect the physiological volume changes of individual small organelles such as mitochondria. (C) 2014 Optical Society of America