Optimization of superovulation induction by human menopausal gonadotropin in guinea pigs based on follicular waves and FSH-receptor homologies
Optimization of superovulation induction by human menopausal gonadotropin in guinea pigs based on follicular waves and FSH-receptor homologies
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DOI：
10.1002/mrd.10242
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发表时间：
2003-02-01
影响因子：
2.5
通讯作者：
Matsuda, J
中科院分区：
文献类型：
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作者：
Suzuki, O;Koura, M;Matsuda, J
豚鼠是研究人类和大多数家畜繁殖的绝佳动物模型，因为与小鼠和大鼠不同，豚鼠经历了一个完整的发情周期。在这项研究中，我们研究了豚鼠在发情周期中卵巢卵母细胞的可用性，以及豚鼠与其他物种之间促卵泡激素（FSH）受体（FSH- r）的同源性，以确定一种有效的促性腺激素和诱导豚鼠超排卵的最佳应用时间。可采集的卵巢卵母细胞数量呈双相变化，在黄体中期和排卵前达到高峰。另一方面，在体外成熟的卵母细胞数量保持不变（类似于10个卵母细胞），直到排卵后第14天，此后逐渐增加。推断的豚鼠FSH-R氨基酸序列与灵长类动物FSH-R的相似性大于与啮齿动物FSH-R的相似性，这表明市售的人类绝经期促性腺激素（hMG）可能是豚鼠超排卵的诱导剂。事实上，在排卵前阶段，hmg处理的豚鼠获得的卵母细胞（5.4+/-1.6，范围0-17，n=10）明显多于自然排卵期(3.6+/-0.1,n=96
The guinea pig represents an excellent animal model for the study of reproduction in humans and most domestic animals because unlike the mouse and rat, it undergoes a complete estrous cycle. In this study, we investigated the availability of ovarian oocytes during the estrous cycle, and the follicle stimulating hormone (FSH) receptor (FSH-R) homologies between guinea pigs and other species, in order to identify an effective gonadotropin and optimal time-of-application for the induction of superovulation in the guinea pig. The number of collectable ovarian oocytes showed biphasic changes with peaks at the midluteal and pre-ovulatory stages. On the other hand, the number of oocytes that matured in vitro remained constant (similar to10 oocytes) until day 14 post-ovulation and increased thereafter. The deduced amino acid sequence of the guinea pig FSH-R showed greater similarity to the primate FSH-R than to the rodent FSH-R, which suggests that commercially available human menopausal gonadotropin (hMG) may be a better inducer of superovulation in guinea pigs. Indeed, significantly more oocytes (5.4+/-1.6, range 0-17, n=10) were obtained from hMG-treated guinea pigs at the pre-ovulatory stage than during spontaneous ovulation (3.6+/-0.1, n=96; P