STRUCTURE AND EXPRESSION OF SUNFLOWER UBIQUITIN GENES
STRUCTURE AND EXPRESSION OF SUNFLOWER UBIQUITIN GENES
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DOI：
10.1007/bf00040634
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发表时间：
1991-09-01
影响因子：
5.1
通讯作者：
TESSIER, LH
中科院分区：
文献类型：
--
作者：
BINET, MN;WEIL, JH;TESSIER, LH
从向日葵基因组文库中分离到两个泛素基因，命名为UbB1和UbB2。它们编码对应于单体的六个重复的多聚泛素转录物。北方印迹分析鉴定了几种不同的转录本大小类别：UbB1和UbB2转录本都存在于最丰富的1.6 kb类别中。与先前分离的在花中以高水平存在的UbF转录物相反，UbB1和UbB2在不同组织中以低水平组成型表达。这两种转录本的水平在热应激后增加。这两个基因表现出很强的同源性，表明它们可能是由复制和转换引起的。令人惊讶的是，UbB1基因显示出与鸡泛素热休克基因的结构相似性，特别是位于第一个ATG前面的内含子的存在。
Two ubiquitin genes, designated UbB1 and UbB2, were isolated from a sunflower genomic library. They encode polyubiquitin transcripts corresponding to six repeats of the monomer. Northern blot analysis identified several different transcript size classes: both UbB1 and UbB2 transcripts are found in the most abundant 1.6 kb class. In contrast to the previously isolated UbF transcript which is present at high levels in flowers, UbB1 and UbB2 are expressed constitutively at low levels in different tissues. The levels of the two transcripts increase after heat stress. The two genes exhibit strong homology suggesting that they may result from duplication and conversion. Surprisingly, UbB1 gene shows structural similarities with the chicken ubiquitin heat shock gene, in particular the presence of an intron located just in front of the first ATG.