Application of DNA typing methods to epidemiology and taxonomy of Candida species.
Application of DNA typing methods to epidemiology and taxonomy of Candida species.
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DNA 分型方法在念珠菌属流行病学和分类学中的应用。
DOI：
10.1128/jcm.25.4.675-679.1987
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发表时间：
1987
影响因子：
9.4
通讯作者：
Stevens,DA
中科院分区：
文献类型：
--
作者：
Scherer,S;Stevens,DA
本文描述了从念珠菌酵母菌中提取DNA的方法，然后对DNA片段进行消化和电泳。所得凝胶图谱（大于100条带）用于假丝酵母分离株的分型。鉴定出四个强条带，其中三个存在于每个分离物中（6至7,3.7或4.2，以及2.5至3千碱基），似乎是编码rRNA的DNA。该方法简便，重现性好。通过多个单一菌落的数百次加倍，这种模式被证明是稳定的。对分离菌株的调查表明，根据凝胶模式的相似性，几个念珠菌可以被划分为相互排斥的类群，并可以建立亚类群。结果表明，这些方法在流行病学和分类学上具有一定的应用价值。DNA分型方法似乎比表型方法具有重要的潜在优势。这些方法为进一步的流行病学研究和技术的进一步发展提供了基础，例如使用克隆探针进行DNA同源性研究。
Methods are described for extraction of DNA from the yeast form of Candida spp., followed by digestion and electrophoresis of DNA fragments. The resulting gel patterns (greater than 100 bands) were used to type Candida isolates. Four intense bands identified, three of which are present in each isolate (6 to 7, 3.7 or 4.2, and 2.5 to 3 kilobases), appear to be DNA encoding the rRNA. The methods proved to be both simple and reproducible. The patterns were shown to be stable through several hundred doublings from multiple single colonies. A survey of isolates showed that, on the basis of similarity of gel patterns, several Candida species could be sorted into mutually exclusive groups, and subgroups could be created. Analyses of this survey suggested the possible epidemiologic and taxonomic applications of these methods. DNA typing methods appear to offer important potential advantages over phenotyping methods. The methods provide a base for further epidemiologic studies and for further development of techniques, such as the use of cloned probes for studies of DNA homology.