Papuamine and haliclonadiamine, obtained from an Indonesian sponge Haliclona sp
Papuamine and haliclonadiamine, obtained from an Indonesian sponge Haliclona sp
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巴布胺和卤氯二胺，取自印度尼西亚海绵 Haliclona sp
DOI：
10.1080/14786419.2012.688050
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
M. Namikoshi
中科院分区：
文献类型：
--
作者：
H. Yamazaki;D. S. Wewengkang;S. Kanno;M. Ishikawa;Henki Rotinsulu;R. E. P. Mangindaan;M. Namikoshi
印度尼西亚海洋海绵Haliclonasp的提取物显示出对人实体癌细胞系MCF-7（乳腺）、LNCap（前列腺）、Caco-2（结肠）和HCT-15（结肠）细胞的有效细胞毒性。细胞核形态学改变和流式细胞术分析表明，提取物中的成分可以诱导这些癌细胞凋亡。生物测定引导分离得到两种五环生物碱，papuamine（1）和haliclonadiamine（2），其抑制六种人类癌细胞系的细胞增殖，IC 50值分别为0.93-1.50和1.00-4.44 µM。化合物1和2使淋巴瘤U937细胞聚集在亚G1期，并诱导染色质浓缩和核碎裂。
The extract of an Indonesian marine spongeHaliclonasp. showed potent cytotoxicity against human solid cancer cell lines, MCF-7 (breast), LNCap (prostate), Caco-2 (colon) and HCT-15 (colon) cells. Study on nuclear morphological changes and flow cytometric analysis suggested that the component(s) in the extract would induce an apoptosis to these cancer cells. Bioassay-guided isolation yielded two pentacyclic alkaloids, papuamine (1) and haliclonadiamine (2), which inhibited cell proliferation of six human cancer cell lines with IC50values of 0.93–1.50 and 1.00–4.44 µM, respectively. Compounds1and2accumulated lymphoma U937 cells at sub-G1phase and induced a condensation of chromatin and fragmentation of nucleus.