Evaluation of type I fusarium head blight resistance of wheat using non-deoxynivalenol-producing fungi
Evaluation of type I fusarium head blight resistance of wheat using non-deoxynivalenol-producing fungi
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DOI：
10.1111/j.1365-3059.2009.02202.x
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发表时间：
2010-02-01
期刊：
影响因子：
2.7
通讯作者：
Nicholson, P.
中科院分区：
文献类型：
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作者：
Gosman, N.;Srinivasachary;Nicholson, P.
研究人员进行了一系列实验，以确定是否可以使用不产生脱氧雪腐镰刀菌醇（DON）的真菌物种/分离株来评估小麦对赤霉病（FHB）的I型抗性（对初始感染的抗性），脱氧雪腐镰刀菌醇是小麦穗部镰刀菌传播的关键真菌毒素。结果表明，非产毒菌nivale Microdochium nivale和M. majus在小麦穗喷雾接种后可以侵染，但在点接种后不能在穗内传播。然而，尽管这些品种可能表现出I型抗性，但它们对小麦的致病性并不高。一种产雪腐镰刀菌毒素（nivalol， NIV）的分离株在喷雾接种后引起了高水平的疾病，但在穗内传播非常缓慢，很少引起接种点以上的白化。研究人员建议，用一种合适的、具有侵袭性的、不产生don的FHB病原体进行喷雾接种，可用于鉴定I型对用产生don的补点接种的抗性，以评估II型抗性（在穗内传播的抗性）。
A series of experiments was conducted to determine whether type I resistance (resistance to initial infection) to fusarium head blight (FHB) in wheat could be assessed using fungal species/isolates that do not produce deoxynivalenol (DON), a mycotoxin critical to the spread of Fusarium graminearum in the wheat spike. It was shown that, while the non-toxin-producing species Microdochium nivale and M. majus could infect following spray inoculation of wheat spikes, they were unable to spread within the spike following point inoculation. However, although these species might reveal type I resistance, they are not highly pathogenic towards wheat. A nivalenol (NIV)-producing isolate of F. graminearum caused high levels of disease following spray inoculation, but spread only very slowly within the spike and rarely induced bleaching above the point of inoculation. It is proposed that spray inoculation with an appropriate, aggressive, non-DON-producing FHB pathogen may be used to characterize type I resistance to complement point inoculation with a DON-producing isolate to assess type II resistance (resistance to spread within the spike).