Photocontrol of Smad2, a multiphosphorylated cell-signaling protein, through caging of activating phosphoserines
Photocontrol of Smad2, a multiphosphorylated cell-signaling protein, through caging of activating phosphoserines
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DOI：
10.1002/anie.200461141
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发表时间：
2004-01-01
影响因子：
16.6
通讯作者：
Muir, TW
中科院分区：
文献类型：
--
作者：
Hahn, ME;Muir, TW
表达蛋白连接已被用于半合成制备Smad2蛋白与笼活化磷酸丝氨酸残基。生物化学和细胞生物学实验表明，笼中的蛋白质在紫外线照射下被激活（见方案）。这为Smad2在活细胞中功能的详细动力学分析奠定了基础。
Expressed-protein ligation has been used for the semisynthetic preparation of a Smad2 protein with caged activating phosphoserine residues. Biochemical and cell biological experiments show that the caged protein is activated upon irradiation with UV light (see scheme). This sets the stage for the detailed kinetic analysis of Smad2 function in living cells.